Project description:LET-418/Mi-2 is an ATP-dependent chromatin remodeler and is part of large complexes like the NuRD complex which are repressive complex and has a role in vulval development in Caenorhabditis elegans. Along with MEP-1, LET-418 also forms the MEC complex which functions in the post embryonic development of the nematode. Earlier studies have shown that there is extensive chromatin remodeling taking place in the intestinal tissue. This study is aimed at deciphering the binding targets of LET-418 and MEP-1 in the intestine using DamID technique. Around 4500 worms were collected from each genotype (let-418::dam, mep-1::dam and gfp::dam) in two replicates. Sequencing and data analysis was performed according to (GOMEZ-SALDIVAR et al. 2021)
Project description:The nematode Caenorhabditis elegans (C. elegans) is often used as a model organism to study cell and developmental biology. Quantitative mass spectrometry has only recently been performed in C. elegans and, so far, most studies have been done on adult worm samples. Here we use quantitative mass spectrometry to characterise protein level changes across the four larval developmental stages (L1-L4) of C. elegans, in biological triplicate. In total, we identify 4,130 proteins and quantify 1,541 proteins that were identified across all four stages in all three biological repeats with at least 2 unique peptides per protein. Using hierarchical clustering and functional ontological analyses, we identify 21 protein groups containing proteins with similar protein profiles across the four stages, and highlight the most overrepresented biological functions in each of these protein clusters. In addition, we use the dataset to identify putative larval stage specific proteins in each individual developmental stage, as well as in the early and late developmental stages. In summary, this dataset provides a system-wide analysis of protein level changes across the four C. elegans larval developmental stages, which serves as a useful resource for the worm development research community.
