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Restorative effect of adipose tissue-derived stem cells (ADSCs) on palmitic acid (PA)-activated IMS/N hepatic stellate cells

ABSTRACT: Restorative effect of adipose tissue-derived stem cells (ADSCs) on palmitic acid (PA)-activated IMS/N hepatic stellate cells

PROVIDER: PRJNA1260916 | ENA |

REPOSITORIES: ENA

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Restorative effect of adipose tissue-derived stem cells (ADSCs) on palmitic acid (PA)-activated IMS/N hepatic stellate cells

Project description:The restorative indirect effect of ADSCs on activated IMS/N hepatic stellate cells was evaluated by gene expression analysis (DNA microarray). IMS/N were stimulated by palmitic acid (PA), and the effect of media derived by cell culture of ADSCs was verified by gene expression analysis.

2025-06-19 | GSE296718 | GEO

Restorative effect of adipose tissue-derived stem cells (ADSCs) on IL17A-activated IMS/N hepatic stellate cells

Project description:The restorative indirect effect of ADSCs on activated IMS/N hepatic stellate cells was evaluated by gene expression analysis (DNA microarray). IMS/N were stimulated by IL17A, and the effect of media derived by cell culture of ADSCs was verified by gene expression analysis.

2025-06-19 | GSE296717 | GEO

Restorative effect of adipose tissue-derived stem cells (ADSCs) on IL17A-activated IMS/N hepatic stellate cells

Project description:Restorative effect of adipose tissue-derived stem cells (ADSCs) on IL17A-activated IMS/N hepatic stellate cells

| PRJNA1260918 | ENA

RNA sequencing to compare gene expession in hepatic stellate cells on a soft (400 Pa) and stiff (25000 Pa) polyacrylamide hydrogels

Project description:Primary human hepatic stellate cells (HSCs) isolated from healthy patients were purchased from Sciencell. They were seeded on either 400 Pa or 25000 Pa polyacrylamide hydrogels for 2 days and collected for RNA isolation. The goal of this study is to determine genes transcriptionally regulated by a stiff matrix. 2 RNA samples of HSCs on 400 Pa and 3 RNA samples of HSCs on 25600 Pa were sent to the University of Minnesota Genomics Center for RNA sequencing.

2017-07-18 | GSE101343 | GEO

The PKR/JNK signalling pathway promotes the palmitic acid-induced myocardial ageing phenotype via SIRT1 inactivation

Project description:This study investigated the role and mechanism of palmitic acid (PA) in ageing phenotype changes in AC16 cells.

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Transcriptional regulation of genes in AML12 cells treated with Palmitic acid, LXR lingand (GW3965) and Ulk1 siRNA

Project description:Transcriptional regulation of genes in AML12 cells treated with Palmitic acid, LXR lingand (GW3965) and Ulk1 siRNA shows differential effect of Ulk1 KD on LXr responsive genes AML-12 cells co-treated with 0.75mM PA+/- 10ÂµM GW3965 for 24 h +/- Ulk1 SiRNA

2016-03-31 | E-GEOD-74059 | biostudies-arrayexpress

RNA sequence for macrodactyly ADSCs

Project description:Macrodactyly is a congenital malformation characterized by enlargement of bone and soft tissues in limbs, typically with excessive accumulation of adipose tissues. Although gain-of-function mutation of PIK3CA has been identified in macrodactyly, the mechanism of PIK3CA mutation in adipose accumulation is poorly understood. In this study, we found that adipocytes from macrodactyly were more hypertrophic than those observed in polydactyly. PIK3CA (H1047R) activating mutation and enhanced activity of PI3K/AKT pathway were detected in macrodactylous adipose-derived stem cells (Mac-ADSCs). To identify the key downstream effectors of PIK3CA activation-mediated adipogenesis in Mac-ADSCs, we examined the transcriptome of Mac-ADSCs, BYL-719 treated Mac-ADSCs and Pol-ADSCs by RNA-Seq analysis.

2020-06-05 | GSE151840 | GEO

Validation of PA-DA probe in mammalian cell and brain lysates

Project description:Here we aim to characterize the palmitic acid diazirine alkyne (PA-DA) probe using chemical proteomics approaches in mammalian cell and mouse brain lysates.

2025-06-24 | PXD051775 | Pride

Expression data from human adipose-derived stem cells (ADSCs)

Project description:Adipose-derived stem cells are angiogenic and attractive for therapeutic angiogenesis. However, ADSCs contain several subsets according to expression pattern of surface markers. We identified CD271 (LNGFR) can isolate ADSCs with superior capacity for angiogenesis. Therefore, we aimed to compare gene expression profile between CD271+ and CD271- ADSCs.

2019-01-14 | GSE125012 | GEO

Palmitic acid alters enhancers and super-enhancers near inflammatory and efferocytosis-associated genes in human monocytes

Project description:Free fatty acids like palmitic acid (PA) are elevated in obesity and diabetes and dysregulate monocyte and macrophage functions, contributing to enhanced inflammation in these cardiometabolic diseases. Epigenetic mechanisms regulating enhancer functions play key roles in inflammatory gene expression, but their role in PA-induced monocyte/macrophage dysfunction is unknown. We found that PA treatment altered the epigenetic landscape of enhancers and super-enhancers (SEs) in human monocytes. Integration with RNA-seq data revealed that PA-induced enhancers/SEs correlated with PA-increased expression of inflammatory and immune response genes, while PA-inhibited enhancers correlated with downregulation of phagocytosis and efferocytosis genes. These genes were similarly regulated in macrophages from mouse models of diabetes and accelerated atherosclerosis, human atherosclerosis, and by infectious agents. PA-regulated enhancers/SEs harbored SNPs associated with diabetes, obesity, and body mass index indicating disease relevance. We verified increased chromatin interactions between PA-regulated enhancers/SEs and inflammatory gene promoters, and reduced interactions at efferocytosis genes. PA-induced gene expression was reduced by inhibitors of BRD4, and NF-kB. PA treatment inhibited phagocytosis and efferocytosis in human macrophages. Together, our results show that PA-induced enhancer dynamics at key monocyte/macrophage enhancers/SEs regulate inflammatory and immune genes and responses. Targeting these PA-regulated epigenetic changes could provide novel therapeutic opportunities for cardiometabolic disorders.

2025-04-23 | GSE281939 | GEO

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