Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of overexpression of aging-association transcription factors on epithelial cells.

ABSTRACT: Effect of overexpression of aging-association transcription factors on epithelial cells.

PROVIDER: PRJNA1261051 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Effect of overexpression of aging-associated transcription factors on epithelial cells.

Project description:RNA-seq profiling of whole, intact uteri at embryonic day 3.5 (E3.5) from young and aged virgin wild-type C57BL/6 mice—each mated with vasectomized wild-type males—identified the transcription factors Foxc1 and Six1 as aging-associated markers. Immunostaining of mouse uteri across an age range of 8 to 41 weeks revealed a progressive upregulation of FOXC1, initially localized to individual stromal cells in the endometrium, followed by prominent expression in both luminal and glandular epithelial cells. To investigate the functional role of Foxc1 and Six1, their full-length open reading frames were cloned into a PiggyBac expression vector, sequence-verified, and overexpressed in Ishikawa cells. Phenotypic characterization and RNA-seq analysis of these cells provided a framework for understanding FOXC1-mediated aging-related effects.

2025-08-07 | GSE296787 | GEO

Master transcription factors in corneal epithelial cells

Project description:Master transcription factors in corneal epithelial cells

| PRJNA280994 | ENA

Overexpression of Sinorhizobium meliloti ECF sigma factors.

Project description:Overexpression of Sinorhizobium meliloti ECF sigma factors.

| PRJNA479836 | ENA

SNAI1 overexpression effect on MCF-10A mammary epithelial cell line

Project description:SNAI1 overexpression effect on MCF-10A mammary epithelial cell line

| PRJNA323452 | ENA

The effect of prohibitin overexpression in Caco2-BBE intestinal epithelial cells

Project description:To determine the effect of prohibitin overexpression on global gene expression in Caco2-BBE intestinal epithelial cells.

2008-09-05 | GSE12605 | GEO

The effect of prohibitin overexpression in Caco2-BBE intestinal epithelial cells

Project description:To determine the effect of prohibitin overexpression on global gene expression in Caco2-BBE intestinal epithelial cells. 4 individual wells of Caco2-BBE cells, passage 41, were transfected with either empty vector (pcDNA4) or prohibitin/pcDNA4 for 72 hours. Total RNA isolated from 4 wells of cells/per treatment were pooled together for labeling and hybridization purposes.

2010-06-19 | E-GEOD-12605 | biostudies-arrayexpress

Functional Characterization of Fission Yeast Transcription Factors by Overexpression Analysis

Project description:In Schizosaccharomyces pombe, over 90% of transcription factor genes are nonessential. Moreover, the majority do not exhibit significant growth defects under optimal conditions when deleted, complicating their functional characterization and target gene identification. Here, we systematically overexpressed 99 transcription factor genes with the nmt1 promoter. Screening the overexpression array revealed that 64 transcription factor genes exhibited reduced fitness when ectopically expressed. Cell cycle defects were also often observed. We further investigated three uncharacterized transcription factor genes (toe1+-toe3+) which displayed cell elongation when overexpressed. Ectopic expression of toe1+ resulted in a G1 delay while toe2+ and toe3+ overexpression produced an accumulation of septated cells with abnormalities in septum formation and nuclear segregation, respectively. Transcriptome profiling and ChIP-chip analysis of the transcription factor overexpression strains indicated that Toe1 activates target genes of the pyrimidine-salvage pathway, while Toe3 regulates target genes involved in polyamine synthesis. We also found that ectopic expression of the putative target genes SPBC3H7.05c, and dad5+ and SPAC11D3.06 could recapitulate the cell cycle phenotypes of toe2+ and toe3+ overexpression, respectively. Furthermore, the phenotypes of toe1+and toe2+ overexpression could be suppressed by deletion of the putative target genes urg2+ and SPAC1399.04c, and SPBC3H7.05c, SPACUNK4.15 and rds1+, respectively. This study implicates new transcription factors and metabolism genes in cell cycle regulation and demonstrates the potential of systematic overexpression analysis to elucidate the function and target genes of transcription factors in S. pombe.

2013-05-11 | GSE46811 | GEO

Effect of NAC154 transcription factor overexpression on the field grown poplar stem wood metabolome

Project description:Several xylem-associated regulatory genes have been identified that control processes associated with wood formation in poplar. Prominent among these are the NAC domain transcription factors (NACs). We evaluated the putative involvement of Populus NAC154, a co-ortholog of the Arabidopsis gene SND2, as a regulator of secondary biosynthetic processes in stem internode tissues by interrogating methanol:water extracts from control and transgenic trees. Comprehensive untargeted metabolite profiling was accomplished with a liquid chromatography-mass spectrometry platform that utilized two different chromatographic supports (HILIC and reversed phase) and both positive and negative ionization modes. Evaluation of current and previous year tissues provided datasets for assessing the effects of NAC154 overexpression in wood maturation processes. Phenolic glycoside levels as well as those of oligolignols, sucrose and arginine were modulated with phenotypic and chemotypic traits exhibiting similar trends. Specifically, increased levels of arginine in the NAC154 overexpressing tissues supports a role for the transcription factor in senescence/dormancy-associated processes.

2015-01-31 | MTBLS119 | MetaboLights

Cut & Run-seq analysis for transcription factors overexpression in GI-MEN cells

Project description:Cut & Run-seq analysis for transcription factors overexpression in GI-MEN cells

| PRJNA887091 | ENA

Critical Role of Runx Transcription Factors in Maintaining Epididymal Epithelial Differentiation

Project description:Critical Role of Runx Transcription Factors in Maintaining Epididymal Epithelial Differentiation

| PRJNA989033 | ENA