Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1262683 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR33566652_1.fastq.gz | Fastqsanger.gz | |||
| SRR33566652_2.fastq.gz | Fastqsanger.gz | |||
| SRR33566653_1.fastq.gz | Fastqsanger.gz | |||
| SRR33566653_2.fastq.gz | Fastqsanger.gz | |||
| SRR33566654_1.fastq.gz | Fastqsanger.gz |
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Project description:Age-dependent changes of the gut-associated microbiome have been linked to increased frailty and systemic inflammation. This study found that age-associated changes of the gut microbiome of BALB/c and C57BL/6 mice could be reverted by co-housing of aged (22 months old) and adult (3 months old) mice for 30-40 days or faecal microbiota transplantation (FMT) from adult into aged mice. This was demonstrated using high-throughput sequencing of the V3-V4 hypervariable region of bacterial 16S rRNA gene isolated from faecal pellets collected from 3-4 months old adult and 22-23 months old aged mice before and after co-housing or FMT.
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Project description:Background: Probiotic-like bacteria treatment has been described to be associated with gut microbiota modifications. Goal: To decipher if the effects of the tested probiotic-like bacteria are due to the bacteria itself or due to the effects of the bacteria on the gut microbiota. Methodology: In this study, gut microbiota has been analyzed from feces samples of subjects with metabolic syndrome and treated with one of the 2 tested probiotic-like bacteria or with the placebo during 3months.
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Project description:Specific pathogen-free laboratory mice are widely used in biomedical research; however, their neonatal-like physiological state often limits their ability to model adult human conditions. Here, we induced adult-like physiological characteristics in laboratory mice by co-housing them with naturally raised mice obtained from pet stores. Co-housing resulted in significant changes in the gut microbiota composition and a marked suppression of tumor growth in multiple tissues. We discovered reduction in Akkermansia muciniphila was a contributing factor to the observed tumor suppression. Furthermore, decreased serum indole metabolite levels were linked to both Akkermansia muciniphila and the tumor-suppressive effects. These findings suggest that Akkermansia muciniphila may, under certain situations, be tumor-promoting and highlight the value of using adult-like mouse models in uncovering critical environmental factors in cancer and other health conditions.
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Project description:We analyzed the effects of antibiotics using a popular model of gut microbiota depletion in mice by a cocktail of antibiotics. We combined intestinal transcriptome together with metagenomic analysis of the gut microbiota to develop a new bioinformatics approach that probes the links between microbial components and host functions. We found that most antibiotic-induced alterations can be explained by three factors: depletion of the microbiota; direct effects of antibiotics on host tissues; and the effects of remaining antibiotic-resistant microbes. While microbe depletion led to down-regulation of immunity, the two other factors primarily inhibited mitochondrial gene expression and amounts of active mitochondria, and induced cell death. By reconstructing and analyzing a transkingdom network, we discovered that these toxic effects were mediated by virulence/quorum sensing in antibiotic-resistant bacteria. This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
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