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Distinct BMP-Smad Signaling Outputs Confer Diverse Functions in Dental Mesenchyme

ABSTRACT: Distinct BMP-Smad Signaling Outputs Confer Diverse Functions in Dental Mesenchyme

PROVIDER: PRJNA1262859 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33476100.fastq.gz	Fastqsanger.gz
	SRR33476095.fastq.gz	Fastqsanger.gz
	SRR33476096.fastq.gz	Fastqsanger.gz
	SRR33476097.fastq.gz	Fastqsanger.gz
	SRR33476098.fastq.gz	Fastqsanger.gz

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Distinct BMP-Smad Signaling Outputs Confer Diverse Functions in Dental Mesenchyme [RNA-seq]

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Distinct BMP-Smad Signaling Outputs Confer Diverse Functions in Dental Mesenchyme [CUT&Run]

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Distinct BMP-Smad Signaling Outputs Confer Diverse Functions in Dental Mesenchyme [CUT&Run]

Project description:The canonical bone morphogenetic protein (BMP) signaling pathway plays a crucial regulatory role in tooth development by activating Smad proteins to regulate gene expression. We previously identified an atypical canonical BMP signaling in dental mesenchyme that is Smad4-independent but Smad1/5-dependent. This study demonstrates that phosphorylated Smad1/5 (pSmad1/5) and Smad4 regulate distinct gene sets in dental mesenchyme. Real-time monitoring of BMP-Smad transcriptional activity revealed that Smad4-dependent canonical BMP signaling is restricted to neurovascular cells surrounding the condensed dental mesenchymal cells where pSmad1/5 is present. Notably, pSmad1/5 in dental mesenchymal cells form complexes with pSmad3 to prevent canonical BMP signaling. CUT&RUN assays revealed genome-wide co-occupancy of pSmad1/5 and pSmad3, indicating their function as transcriptional regulation units. Integrative analyses demonstrated that this atypical canonical BMP signaling regulates tooth sensory innervation and maintains odontogenic inductive potential in dental mesenchyme, enabling the identification of critical genes for maintaining tooth inductive capability. Our findings elucidate the operating mechanism of atypical canonical BMP signaling in dental mesenchymal cells and clarify how BMP-Smad signaling exerts diverse functions across different cell types, informing future tooth bioengineering strategies.

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Distinct BMP-Smad Signaling Outputs Confer Diverse Functions in Dental Mesenchyme

Project description:This SuperSeries is composed of the SubSeries listed below.

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Quantitative proteomics of the SMAD transcription factor family reveals IPO5 as a BMP Receptor-SMAD specific importin

Project description:Gene-specific transcription factors (GSTFs) control of gene transcription by DNA binding and specific protein complex recruitment, which regulates promoter accessibility for transcription initiation by RNA polymerase II. GSTFs that are frequently mutated in colon and rectal carcinomas are Suppressor of Mothers Against Decapentaplegic 2 (SMAD2) and SMAD4, which play an important role in the TGF-β signaling pathways controlling cell fate and proliferation (ref.). The SMAD protein family is a diverse and it can be divided into three subclasses: receptor activated SMADs, inhibitory SMADs and the common SMAD4 co-activator. To study protein interactors of the SMAD protein family we generated a quantitative proteomics pipeline that allows for inducible expression of GFP-tagged SMAD proteins followed by affinity purification and MS analysis. The nuclear importin IPO5 was identified as a novel interacting protein of SMAD1. Overexpression of IPO5 shows forced BMP R-SMAD nuclear localization confirming a functional relationship between BMP but not TGF-β R-SMADs and IPO5. Finally we provide evidence that the length of the lysine stretch in the NLS is involved in importin selection.

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