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OTTR ribosome profiling in Mettl14 knockout mESC

ABSTRACT: OTTR ribosome profiling in Mettl14 knockout mESC

PROVIDER: PRJNA1262973 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33565247.fastq.gz	Fastqsanger.gz
	SRR33565248.fastq.gz	Fastqsanger.gz
	SRR33565249.fastq.gz	Fastqsanger.gz
	SRR33565250.fastq.gz	Fastqsanger.gz
	SRR33565251.fastq.gz	Fastqsanger.gz

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OTTR ribosome profiling in Mettl14 knockout mESC

Project description:Degradation of mRNA containing N6-methyladenosine (m6A) is essential for cell growth, differentiation, and stress responses. Here, we show that m6A markedly alters ribosome dynamics and that these alterations mediate the degradation effect of m6A on mRNA. We find that m6A is a potent inducer of ribosome stalling, and these stalls lead to ribosome collisions that form a unique conformation unlike those seen in other contexts. We find that the degree of ribosome stalling correlates with m6A-mediated mRNA degradation, and increasing the persistence of collided ribosomes correlates with enhanced m6A-mediated mRNA degradation. Ribosome stalling and collision at m6A is followed by recruitment of YTHDF m6A reader proteins to promote mRNA degradation. We show that mechanisms that reduce ribosome stalling and collisions, such as translation suppression during stress, stabilize m6A-mRNAs and increase their abundance, enabling stress responses. Overall, our study reveals the ribosome as the initial m6A sensor for beginning m6A-mRNA degradation.

2025-05-19 | GSE297194 | GEO

TimeLapse-seq after translation inhibition in Mettl14 knockout mESC

Project description:TimeLapse-seq after translation inhibition in Mettl14 knockout mESC

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Active ribosome profiling in SETD2 or METTL14 knockdown HepG2 cells

Project description:SETD2 is the specific methyltransferase of H3K36me3, while METTL14 is a critical subunit of the m6A methyltransferase complex. To evaluate the effect of SETD2 and METTL14 on translation, we conducted robosome profiling in SETD2 and METTL14 knockdown and control HepG2 cells. Our RNA ribosome profiling revealed that depletion of SETD2 and METTL14 resulted in a global reduction in RNA translation and the changes of translation efficiency were correlated between SETD2 and METTL14 knockdown cells.

2019-02-21 | GSE121952 | GEO

Ribosome profiling of ABCE1-knockout cells

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Active ribosome profiling in SETD2 or METTL14 knockdown HepG2 cells

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Ribosome profiling on heat shocked wild type and Mettl14 -/- mES cells

Project description:Ribosome profiling on heat shocked wild type and Mettl14 -/- mES cells

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Ribosome profiling of wild-type and NSUN2 knockout flies

Project description:We performed ribosome profiling for wild-type and NSUN2 knockout flies.

2023-10-30 | GSE160564 | GEO

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2025-05-15 | GSE297221 | GEO

METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (mRNA)

Project description:METTL3 and METTL14 are two components that form the core heterodimer of the main RNA m6A methyltransferase complex (MTC, also known as m6A writer) that installs m6A. Surprisingly, depletion of METTL3 or METTL14 displayed distinct effects on mouse embryonic stem cell (mESC) self-renewal. While comparable global hypo-methylation in RNA m6A was observed in Mettl3 or Mettl14 knockout mESCs, respectively. Mettl14 knockout led to a globally decreased nascent RNA synthesis, whereas Mettl3 depletion resulted in transcription upregulation, suggesting that METTL14 might possess an m6A-indenepent role in gene regulation. We found that METTL14 colocalizes with the repressive H3K27me3 modification and PRC2 complex. Mechanically, METTL14, but not METTL3, recognizes H3K27me3 and recruits KDM6B to induce H3K27me3 demethylation independent of METTL3. Depletion of METTL14 thus led to a global increase in H3K27me3 level along with a global gene suppression . The regulation of H3K27me3 by METTL14 is essential to the transition of mESCs from self-renewal to differentiation. This work reveals a regulation mechanism on heterochromatin by METTL14 in a manner distinct from METTL3 and independently of m6A, and critically impacts transcriptional regulation, stemness maintenance and differentiation of mESCs.

2024-08-16 | GSE183400 | GEO

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