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ABSTRACT: Indoor Synechococcus sp.PCC 7002 Symbiotic Microbial Community

PROVIDER: PRJNA1264977 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33620018_1.fastq.gz	Fastqsanger.gz
	SRR33620018_2.fastq.gz	Fastqsanger.gz
	SRR33620019_1.fastq.gz	Fastqsanger.gz
	SRR33620019_2.fastq.gz	Fastqsanger.gz

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Photophysiological and Genetic Factors Underlying Fast Photosynthetic Growth of Unicellular Cyanobacterium Synechococcus sp. PCC 7002

Project description:Application of genome-scale 'omics approaches to dissect subcellular pathways and regulatory networks governing the fast-growing response of Synechococcus sp. PCC 7002 response to variable irradience levels. We employed controlled cultivation and next-generation sequencing technology to identify transcriptional responses of euryhaline unicellular cyanobacterium Synechococcus sp. PCC 7002 grown under steady state conditions at six irradiance levels ranging from 33 to 760 µmol photons m-2 sec-1.

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Project description:We calculated global RNA half lives for all genes in the cyanobacteria Synechococcus sp. strain PCC 7002. Samples from three replicates of the WT strain were taken before and following the addition of the antibiotic rifampicin to stop nascent transcription. RNA spike-ins were added for normalization and using the number of RNA-sequencing reads we were able to calculate half lives for genes, transcripts, and for each position on the genome.

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