ABSTRACT: Transcriptomic profiling of Eurasian perch (Perca fluviatilis) larvae obtained with cryopreserved sperm used shortly after thawing and after 30 min post-thaw storage
Project description:Determining the physiological effects of parasites and characterizing genes involved in host responses to infections are essential to improving our understanding of host-parasite interactions and their ecological and evolutionary consequences. This task, however, is complicated by high diversity and complex life histories of many parasite species. The use of transcriptomics in the context of wild-caught specimens can help ameliorate this by providing both qualitative and quantitative information on gene expression patterns in response to parasites in specific host organs and tissues. Here, we evaluated the physiological impact of the widespread parasite, the pike tapeworm (Triaenophorus nodulosus), on its second intermediate host, the Eurasian perch (Perca fluviatilis).
2024-07-04 | GSE237655 | GEO
Project description:Gut microbiome of Eurasian perch (perca fluviatilis L.)
| PRJNA1193834 | ENA
Project description:Transcriptomic profiling of Eurasian perch (Perca fluviatilis) larvae obtained with either fresh or cryopreserved sperm
| PRJNA1073726 | ENA
Project description:Transcriptomic profiling of Eurasian perch (Perca fluviatilis) larvae obtained with either fresh or cryopreserved sperm
| PRJNA1073695 | ENA
Project description:Genomics of humic adaptation in Eurasian perch (Perca fluviatilis)
Project description:This study investigates the impact of cryopreservation and freezing media on RNA quality and gene expression profiles of peripheral blood mononuclear cells (PBMC). No significant difference in cell viability or RNA quality was observed between freshly isolated and cryopreserved cells, even after two freeze-thaw cycles. Transcriptome analysis revealed no significant differences in alignment rates or read counts across various conditions. Differential gene expression analysis identified changes between fresh and thawed samples, with a consistent downward trend of mRNA abundance in the frozen samples, but minimal changes were observed between the first and second freeze-thaw cycles. Gene set enrichment analysis showed only minor significant differences, and stress-related gene sets were not upregulated. Freezing medium appears to be the safer option compared to freezing in trizol. These results confirm that cryopreservation and thawing processes do not compromise RNA integrity or sequencing reliability in PBMC, supporting the use of cryopreserved samples for gene expression studies.
