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ABSTRACT: Viral metagenome with PE MPs

PROVIDER: PRJNA1265959 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33659128_1.fastq.gz	Fastqsanger.gz
	SRR33659128_2.fastq.gz	Fastqsanger.gz
	SRR33659129_1.fastq.gz	Fastqsanger.gz
	SRR33659129_2.fastq.gz	Fastqsanger.gz
	SRR33659130_1.fastq.gz	Fastqsanger.gz

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Proteomic insights into composition-dependent effects of microplastics on freshwater microalgae Chlamydomonas reinhardtii

Project description:The widespread presence of microplastics (MPs) in freshwater systems has raised concerns about their potential ecotoxicity on aquatic organisms. In this study, we evaluated the effects of four MPs with different compositions, namely polyethylene (PE), polystyrene (PS), polyvinyl chloride (PVC) and polypropylene (PP), on freshwater microalgae Chlamydomonas reinhardtii. PS and PVC MPs caused greater growth inhibition and stronger oxidative stress response than PP and PE MPs. Proteomics analysis was employed to explore the mechanical understanding of the composition-dependent toxicity of MPs on microalgae. Proteins involved in photosynthesis processes were identified as contributors to the diverse responses of microalgae to differently composed MPs. Photosynthesis activity of algae, including pigment content and photoprotective response, was determined to reflect the distinct effect of the four MPs. The indicated down-regulated expression of photosynthetic proteins by proteomics analysis was further confirmed using western blot, with PVC and PS showing greater impacts on their expression reduction. Our findings not only show the composition-dependent effect of MPs on microalgae but also provide important insights into the molecular mechanism of MPs’ toxicity on natural phytoplankton species.

2024-06-06 | PXD052887 |

Neurofibromin (Nf1) controls metabolic balance and Notch-dependent quiescence of murine juvenile myogenic progenitors [ChIP-seq]

Project description:We conclude that Nf1Myf5 MPs are driven towards quiescence and show metabolic reprogramming with severely inhibited glycolytic metabolism that, in turn impinges on H4K16ac decoration and expression of myogenic differentiation-related genes. ChIP-Seq analysis of freshly isolated p7 MPs showed apparently unaltered H3K4me3 levels between controls and Nf1Myf5 MPs, while H3K27me3 levels were globally reduced in Nf1Myf5 MPs. A reduction of H3K27me3 decoration was observed at genes associated with quiescence as Pax7, or with cell cycle repression as Cdkn2a. The assumption of a quiescent state in Nf1Myf5 MPs is concomitant to decreased repressive H3K27me3 modification at quiescence-associated genes.

2021-09-25 | GSE159022 | GEO

Microarray experiment of murine macrophages co-cultured with neural stem cells in the presences of LPS

Project description:This experiment aimed at characterising the modulatory role of murine induced Neural Stem Cells (iNSCs) and Neural Stem Cells (NSCs) on macrophages (MPs) exposed to LPS in vitro. NaÃ¯ve MPs were polarized into an M1-like phenotype with LPS, and then co-cultured with 1:1 ratios of iNSCs in a trans-well system that avoids cell-to-cell contacts. NaÃ¯ve MPs, LPS-stimulated MPs and LPS-stimulated MPs co-cultured with NSCs were used as controls.

2018-01-01 | E-MTAB-5579 | biostudies-arrayexpress

Microarray analysis of Normal and MPS VII mouse aorta

Project description:Mucopolysaccharidosis VII (MPS VII) is due to mutations within the gene encoding the lysosomal enzyme beta-glucuronidase, and results in the accumulation of glycosaminoglycans. MPS VII causes aortic dilatation and elastin fragmentation. In this study we performed microarray analysis of ascending aortas from normal and MPS VII mice, trying to find out possible genes responsible for the phenotype observed. In addition, during our breeding strategy, we noticed that some MPS VII mice had less dilated aortas, and we proposed that an yet-unidentified gene could be responsible for the difference observed. We therefore included in the analysis two MPS VII mice with aortas that were not dilated. Total RNA extracted from ascending aortas from 3 Normal mice, 3 MPS VII mice with dilated aortas and 2 MPS VII mice with aortas that were not dilated.

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Defining the Secrebiome: the expressed secretome of the gut microbiome and its role in obese Mexican children

Project description:high throughput sequencing of the metagenome of mexican children

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Project description:mps

| PRJNA397169 | ENA

Co-exposure to Polyethylene Fiber and Salmonella enterica Typhimurium Alters Microbiome and Metabolome of in vitro Chicken Cecal Mesocosms

Project description:Humans and animals encounter a summation of exposures during their lifetime (the exposome). In recent years, the scope of the exposome has begun to include microplastics. Microplastics (MPs) have increasingly been found in locations where there could be an interaction with Salmonella enterica Typhimurium, one of the commonly isolated serovars from processed chicken. In this study, the microbiota response to a 24-hour co-exposure to Salmonella enterica Typhimurium and/or low-density polyethylene (PE) microplastics in an in vitro broiler cecal model was determined using 16S rRNA amplicon sequencing (Illumina) and untargeted metabolomics. Community sequencing results indicated that PE fiber with and without S. Typhimurium yielded a lower Firmicutes/Bacteroides ratio compared to other treatment groups, which is associated with poor gut health, and overall had greater changes to the cecal microbial community composition. However, changes in the total metabolome were primarily driven by the presence of S. Typhimurium. Additionally, the co-exposure to PE Fiber and S. Typhimurium caused greater cecal microbial community and metabolome changes than either exposure alone. Our results indicate that polymer shape is an important factor in effects resulting from exposure. It also demonstrates that microplastic-pathogen interactions cause metabolic alterations to the chicken cecal microbiome in an in vitro chicken cecal model.

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Gene expression analysis of brains of MPSIIIB mice

Project description:Gene expression analysis of brain samples of wt MPS IIIB mice and MPS IIIB mice treated with AAV-NAGLU

2022-12-01 | E-MTAB-2984 | biostudies-arrayexpress

Immune-mediated Inflammation May Contribute to the Pathogenesis of Cardiovascular Disease in Mucopolysaccharidosis Type I

Project description:Comparisons of canine arterial gene expression between control and untreated MPS animals were conducted with a canine-specific microarray covering 43,803 probes (Agilent G2519F 4x44k, Santa Clara, CA), for a total of four comparison groups: MPS ascending aorta vs. control ascending aorta, MPS descending aorta vs. control descending aorta, MPS carotid artery vs. control carotid artery, and finally pooled MPS artery (ascending aorta, descending aorta, carotid artery) vs. pooled control artery. Each comparison used four pairs of MPS vs corresponding age- and gender- matched animals to produce four biologic replicates.

2016-03-05 | GSE78889 | GEO

Expression data of Normal versus Mutant MPS VII C3H mouse

Project description:We used microarray to detect pathway differences in the various brain regions in a monogenic in mucopolysaccharidosis type VII ( MPS VII ), a mouse model of a lysosomal storage disease A number of changes revealed unexpected system and process alterations, such as upregulation of the immune system with few inflammatory changes (a significant difference from the closely related MPS IIIb model), down-regulation of major oligodendrocyte genes even though white matter changes are not a feature histopathologically, and a plethora of developmental gene changes. 94 samples, no replicates, made up of half normals and half MPS mutant mice for the MPS VII mutation backcrossed on a C3h-heouj background

2012-12-01 | E-GEOD-34071 | biostudies-arrayexpress

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