ABSTRACT: Biofilm bacteria communities in oligotrophic headwater streams of Patagonia: 16S rRNA metabarcoding across climate, stream order and forest intervention
Project description:Biofilm bacteria communities in oligotrophic headwater streams of Patagonia: 16S rRNA metabarcoding across climate, stream order and forest intervention
Project description:Prolific heterotrophic biofilm growth is a common occurrence in airport receiving streams containing deicer and anti-icer runoff. This study investigated relations of heterotrophic biofilm prevalence and community composition to environmental conditions at stream sites upstream and downstream of Milwaukee Mitchell International Airport in Milwaukee, WI, during two deicing seasons (2009–2010 and 2010–2011). Modern genetic tools (such as microarray) have not previously been applied to biofilm communities in this type of setting. We used microarray results to characterize biofilm community composition as well as the response of the biofilm community to environmental factors (i.e., organic content (using chemical oxygen demand concentration) and temperature).
2019-05-13 | GSE129990 | GEO
Project description:Metabarcoding of stream biofilm eukaryotes
Project description:C2C12 myoblasts were infected with a retrovirus expressing Pax7d or with an empty virus (puro) as a control. All of the samples originated from the same common pool of parental C2C12. This pool was split into six streams. A single prep of Pax7d-puro virus was split into three volumes and used to infect three of the streams. A single prep of puro-alone virus was similarly split in three and used to infect the remaining three streams. From the point of the infection forward each stream was maintained distinct from the others. Cells were infected and grown simultaneously under identical conditions. The puro samples may be reused as controls for future experiments, and therefore were immediately hybridized to the MOE430A and MOE430B chips in order to generate a complete dataset. The Pax7d samples, as a specific component of this experiment, were only hybridized to the MOE430A chip as a first-look into the system. Experiment Overall Design: this experiment include 2 samples and 9 replicates
Project description:Stress gene expression profiling of hepatic tissue in wild caught juvenile coho from perenial streams. Stream locations were based on a gradient of urban impact
2018-06-13 | GSE115688 | GEO
Project description:Metabarcoding stream macroinvertebrates and eDNA samples from streams in southern Ontario
