Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Testicular and epididymal samples of mice infected with SFTSV

PROVIDER: PRJNA1267468 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR33691333_1.fastq.gz	Fastqsanger.gz
	SRR33691333_2.fastq.gz	Fastqsanger.gz
	SRR33691335_1.fastq.gz	Fastqsanger.gz
	SRR33691335_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 23

Similar Datasets

Homo sapiens

Project description:SFTSV infected samples

| PRJNA542189 | ENA

Severe Fever with Thrombocytopenia Syndrome Virus Induces Lactylation of m6A Reader Protein YTHDF1 to Facilitate Viral Replication

Project description:To investigate the role of m6A modification during SFTSV infection, we established a SFTSV-infected cell model, in which each m6A modification change was identified.

2024-12-18 | GSE256528 | GEO

Ferret animal model of severe fever with thrombocytopenia syndrome phlebovirus for human lethal infection and pathogenesis

Project description:Severe fever with thrombocytopenia syndrome phlebovirus (SFTSV), listed in the WHO most dangerous pathogens, has 12-30% fatality rates with a characteristic thrombocytopenia syndrome. With a majority of clinically diagnosed SFTSV patients older than ~50 years, age is a critical risk factor for SFTSV morbidity and mortality. Here, we report an age-dependent ferret model of SFTSV infection and pathogenesis that fully recapitulates the clinical manifestations of human infections. While young adult ferrets (≤2 years old) did not show any clinical symptoms and mortality, SFTSV-infected aged ferrets (≥4 years old) demonstrated severe thrombocytopenia, reduced white blood cells, and high fever with 93% mortality rate. Moreover, significantly higher viral load was observed in aged ferrets. Transcriptome analysis of SFTSV-infected young ferrets revealed strong interferon-mediated anti-viral signaling, whereas inflammatory immune responses were markedly upregulated and persisted in aged ferrets. Thus, this immunocompetent age-dependent ferret model should be useful for anti-SFTSV therapy and vaccine development.

2018-10-30 | GSE121911 | GEO

Immunoprecipitation-mass spectrometry and proximity labeling-mass spectrometry studies on CFAP91

Project description:Testicular lysates from WT and Cfap91-BioID2-3xFLAG mice were immunoprecipitated by an anti-FLAG antibody to determine CFAP91-interacting proteins. Cauda epididymal spermatozoa were isolated from WT and Cfap91-BioID2-3xFLAG mice and incubated with biotin (+) medium. Then, biotinylated proteins were pulled down by streptavidin to determine the proximate proteins of CFAP91.

2025-08-12 | PXD065243 | JPOST Repository

Project description:Transcriptomic analysis of SFTSV-infected THP-1 samples

| PRJNA889171 | ENA

Project description:SFTSV-infected BMDMs

| PRJNA1086126 | ENA

Project description:SFTSV-infected THP-1

| PRJNA1086175 | ENA

A longitudinal sampling study of transcriptomic and epigenetic profiles in patients with thrombocytopenia syndrome

Project description:Severe fever with thrombocytopenia syndrome (SFTS) is a new tick-borne infectious disease caused by a new SFTS virus (SFTSV). Due to its 12%-50% high fatality rate and the possibility of pandemic transmission, as well as no specific antiviral drugs for the treatment so far, SFTSV has been listed as one of the top 10 priority infectious diseases by the World Health Organization. Currently, there are rare studies of transcriptomic analysis for the patients infected with SFTSV, which makes it difficult to deeply understand the life cyle and pathogenicity of this virus. To explore the differences of transcripts after SFTSV infection, we performed a longitudinal sampling study to systematically investigate the chronological changes of viral load and transcriptomic and epigenetic characterization using white blood cells from SFTSV patients. The results showed significant changes in the expression of some genes from onset to recovery of SFTSV infection. Moreover, these differentially expressed genes showed good consistency in the three patients at different stages of treatment, which may contribute to the pathophysiology of SFTSV and thus lead to a breakthrough in SFTSV therapy. Moreover, by m6A-seq, we found some genes that might be regulated by m6A. This study in transcript changes and RNA modification may open a brand new direction to our understanding of SFTSV and play an important role in the drugs discovery for effective treatment.

2021-08-05 | GSE165518 | GEO

Quantitative proteomic analysis of SFTSV infected THP-1 cells

Project description:Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne virus causing a high fatality of 12-50% in infected patients. In-depth understanding of the SFTSV induced pathogenesis mechanism is critical for developing effective anti-SFTS therapeutics.

2022-09-05 | PXD017224 | Pride

Epididymal acquired sperm microRNAs modify post-fertilization embryonic gene expression

Project description:Sperm small RNAs have emerged as important non-genetic contributors to embryogenesis and offspring health. A subset of sperm small RNAs are thought to be acquired during epididymal transit. However, the transfer of RNAs from the somatic epididymis to the sperm has been questioned, and the identity of the specific small RNAs transferred remains unclear. Here, we employ Cre/Lox genetics to generate germline- and epididymal-specific Dgcr8 conditional knockout mice to investigate the dynamics of sperm microRNAs and their function in the early embryo. Interestingly, sperm from germline specific Dgcr8 knockout males restored the levels of 58 of the 98 (59%) miRNAs that were lost in testicular sperm during epididymal transit. Conversely, sperm from epididymal Dgcr8 knockouts displayed a 5-fold reduction in 25 miRNAs. This substantial loss of epididymal miRNAs in sperm was accompanied by transcriptomic changes in the embryo which was rescued by microinjection of epididymal miRNAs. These findings ultimately demonstrate the acquisition of miRNAs by sperm during epididymal transit and their regulation of post-fertilization embryonic gene expression.

2024-08-31 | GSE244885 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data