Rumen microbiota
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1267572 | ENA |
REPOSITORIES: ENA
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Project description:A healthy rumen is crucial for normal growth and improved production performance of ruminant animals. Rumen microbes participate in and regulate rumen epithelial function, and the diverse metabolites produced by rumen microbes are important participants in rumen microbe-host interactions. SCFAs, as metabolites of rumen microbes, have been widely studied, and propionate and butyrate have been proven to promote rumen epithelial cell proliferation. Succinate, as an intermediate metabolite in the citric acid cycle, is a final product in the metabolism of certain rumen microbes, and is also an intermediate product in the microbial synthesis pathway of propionate. However, its effect on rumen microbes and rumen epithelial function has not been studied. It is unclear whether succinate can stimulate rumen epithelial development. Therefore, in this experiment, Chinese Tan sheep were used as experimental animals to conduct a comprehensive analysis of the rumen microbiota community structure and rumen epithelial transcriptome, to explore the role of adding succinate to the diet in the interaction between the rumen microbiota and host.
2023-06-12 | GSE233696 | GEO
Project description:The rumen plays an essential role in ruminant digestion, absorption, metabolism, and health. The rumen undergoes substantial changes in size and function from birth to adulthood. The molecular mechanisms underlying these changes are not fully understood. This study was aimed to identify the transcription factors and signaling pathways mediating these changes in cattle. We used 6 neonatal bull calves and 6 adult steers, all Angus-crossbred, in this study. We found that the ratios of the emptied rumen, reticulum, omasum, and abomasum to total body weight in adult steers were 4.8 (P < 0.01), 3.1 (P < 0.01), 6.0 (P < 0.01), and 0.8 (P = 0.9) times those in neonatal calves, respectively. Histological examinations showed a 7.4-, 2.0-, 3.0-, 2.9-, and 4.6-fold increase (P < 0.01 for all) in the length of the rumen papillae and the thickness of the rumen epithelium, tunica mucosa and submucosa, tunica muscularis, and tunica serosa, respectively, from neonatal calves to adult steers. Papilla density was lower in adult steers than in neonatal calves (P < 0.05). The size of the rumen epithelial cells was not different between neonatal calves and adult steers (P = 0.57). RNA sequencing identified 2,906 genes differentially expressed (adjusted P < 0.05 and |log2fold change| ≥ 1) in the rumen between neonatal calves and adult steers, of which 1,586 were downregulated and 1,320 upregulated in adult steers. Bioinformatic analyses indicated organ development and morphogenesis, neuronal differentiation, blood vessel development, Ras signaling, and Wnt signaling were among the functional terms enriched in genes downregulated in adult steers, while fatty acid metabolism, ketogenesis, immune responses, PPAR signaling, and Rap1 signaling were among those enriched in genes upregulated in adult steers. Bioinformatic analyses also indicated that SRF, IRF4, SPI1, ETS1, and PURA were the major transcription factors mediating gene upregulation and that TCF4, MESP1, TCF3, ID4, and SNAI2 were the major transcription factors mediating gene downregulation in the rumen from neonatal calves to adult steers. Taken together, these results suggest that the rumen grows by increasing the number, not by the size, of individual cells from birth to adulthood, that the absorptive, metabolic, immune, and motility functions of the rumen are attained or enhanced during the postnatal life, and that the changes in rumen size and function from birth to adulthood are mediated by many transcription factors including SRF and TCF4 and many signaling pathways including the PPAR and Wnt signaling pathways.
2025-02-05 | GSE280816 | GEO
Project description:Investigation of whole genome gene expression level changes in rumen epithelium of dairy cattle at different stages of rumen development and on different diets.
2009-11-01 | GSE18382 | GEO
Project description:We performed single-cell RNA-sequencing on the rumen epithelium of dairy cows to construct an epithelial single-cell map of the rumen.
2021-10-21 | GSE175652 | GEO
Project description:As the unique organ, rumen plays vital roles in providing products for humans, however, the underlying cell composition and interactions with epithelium-attached microbes remain largely unknown. Herein, we performed an integrated analysis in single-cell transcriptome, epithelial microbiome, and metabolome of rumen tissues to explore the differences of microbiota-host crosstalk between newborn and adult cattle models. We found that fewer epithelial cell subtypes and more abundant immune cells (e.g., Th17 cells) in the rumen tissue of adult cattle. Metabolism-related functions and oxidation-reduction process were significantly upregulated in the adult rumen epithelial cell subtypes. The epithelial Desulfovibrio was significantly enriched in the adult cattle. To further clarify the role of Desulfovibrio in host’s oxidation-reduction process, we performed metabolomics analysis of rumen tissues and found that Desulfovibrio showed a high co-occurrence probability with the pyridoxal in the adult cattle compared with newborn ones. The adult rumen epithelial cell subtypes also showed stronger ability of pyridoxal binding. These indicates that Desulfovibrio and pyridoxal likely play important roles in maintaining redox balance in adult rumen. The integrated analysis provides novel insights into the understanding of rumen function and facilitate the future precision improvement of rumen function and milk/meat production in cattle.
2022-11-17 | GSE183285 | GEO
Project description:Metaproteomic studies of the rumen microbiota are challenged by the need of optimized sample preparation protocols in order to retrieve an enhanced amount of prokaryotic instead of plant and bovine derived cells before protein extraction and subsequent LC-MS/MS analysis. The present study evaluates three different protocols applied to the rumen microbiota either attached to plant fibres or present as planktonic cells. The findings of our work suggest the integration of cheesecloth-gauze filtration in sample preparation to achieve a better protein identification ratio.
2015-04-01 | PXD001526 | Pride
Project description:The objective of our study was to assess the effect of rumen-protected niacin supplementation on the overall transcriptomics profile of liver tissue on growing Angus × Simmental steers and heifers. Consequently, the vasodilatory, detoxifying, and immune suppressor effects of niacin were evaluated in hepatocytes. After a 30-day supplementation period with rumen-protected niacin on normal weaned beef calves, we observed a significant list of benefits at the liver transcriptome level. Several metabolic pathways revealed positive effects of administration of rumen-protected niacin; for example, a decrease in lipolysis, apoptosis, inflammatory responses, atherosclerosis, oxidative stress, fibrosis, and vasodilation-related pathways. Therefore, results from this study could potentially promote supplementation of rumen-protected niacin on beef cattle backgrounding operations or new arrivals to a feedlot, especially during the acclimation period when the health status of growing beef cattle is usually compromised.
2025-06-19 | GSE206468 | GEO
Project description:The time-resolved impact of monensin on the active rumen microbiome in a rumen-simulating technique (Rusitec) was studied with metaproteomic and metabolomic approaches. Upon monensin treatment, decreased catabolism linked to fiber degradation was observed by the reduced abundance of proteins assigned to fibrolytic bacteria and glycoside hydrolases, sugar transporters and carbohydrate metabolism. Reduced amounts of ammonium as well as branched-chain fatty acids pointed towards a decreased proteolytic activity. The family Prevotellaceae exhibited increased resilience in the presence of monensin, with a switch of metabolism from acetate to succinate production. Prevotella species harbor a membrane bound electron transfer complex, which drives the reduction of fumarate to succinate, the substrate for propionate production in the rumen habitat. Besides the increased succinate production, a concomitant depletion of methane concentration was observed upon monensin exposure. Our study demonstrates that Prevotella sp. shifts its metabolism successfully in response to monensin exposure and Prevotellaceae represents the key bacterial family stabilizing the rumen microbiota during exposure to monensin.
2022-12-11 | PXD034099 | Pride