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ABSTRACT: A high-quality chromosome-level genome assembly and annotation of the giant freshwater prawn (Macrobrachium rosenbergii)

PROVIDER: PRJNA1268154 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33733770_subreads.fastq.gz	Fastqsanger.gz
	SRR33733771_1.fastq.gz	Fastqsanger.gz
	SRR33733771_2.fastq.gz	Fastqsanger.gz
	SRR33733772_1.fastq.gz	Fastqsanger.gz
	SRR33733772_2.fastq.gz	Fastqsanger.gz

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Gut microbiomes associated with hepatopancreatic and gonadal transcriptomes and their impact on growth traits in M. rosenbergii

Project description:With the increasing global demand for high-quality protein resources, improving the feed utilization rate and growth rate of aquaculture animals is the main way to solve the shortage of high-quality animal protein for humans. Improving the growth rate of Macrobrachium rosenbergii, the world's second most-produced freshwater prawn, is of particular importance for increasing the supply of high-quality protein to humans. In this study, we found that growth traits, such as body weight and weight gain of M. rosenbergii, were associated with gut microbes by applying 16s sequencing technology, as well as associated with hepatopancreatic and gonadal transcriptomes. By comparing the relative abundance of gut microbes between the male (high weight gain, HiWG) and female (low weight gain, LoWG) animals, we have shown that at the phyla, family and genera level, the abundance of microbes existed significantly difference between both groups. At the genera level, the abundance of Candidatus Hepatoplasma and unidentified Chloroplast lower in the LoWG animals than in the HiWG animals, and Lactobacillus and Ligilactobacillus have opposed trend. The abundance of Candidatus Hepatoplasma and unclassfied Chloroplast was associated with the growth traits in the HiWG group, as well as with the expression of glutathione peroxidase and collagen, which were highly expressed in the hepatopancreas and gonads of HiWG group animals and significantly associated with protein digestion, absorption and metabolism of M. rosenbergii and might promote growth and development. These associations were not detected in the female LoWG group.

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Project description:Female Giant freshwater prawn, Macrobrachium rosenbergii, Transcriptomes

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Project description:Transcriptome analysis of the giant freshwater prawn Macrobrachium rosenbergii

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Project description:The nursery microecology of giant freshwater prawn (Macrobrachium rosenbergii) dynamics

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Identification and characterization analysis of the Oriental River Prawn (Macrobrachium nipponense) microRNA response to hypoxia using a deep sequencing approach [miRNA-Seq]

2016-06-01 | GSE72417 | GEO

Identification and characterization analysis of the Oriental River Prawn (Macrobrachium nipponense) microRNA response to hypoxia using a deep sequencing approach [miRNA microarray analysis]

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Project description:Complete Mitochondrial Genome Sequence of Giant Freshwater Prawn, Macrobrachium rosenbergii of Bangladesh

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Identification and characterization analysis of the Oriental River Prawn (Macrobrachium nipponense) microRNA response to hypoxia using a deep sequencing approach [miRNA-Seq]

Project description:MicroRNAs (miRNAs) function as regulators in a broad range of phenotypes. The Oriental River Prawn (Macrobrachium nipponense) is an important commercial species that is widely distributed in freshwater and low-salinity estuarine regions of China and other Asian countries. To date, there are no reports describing M. nipponense miRNAs. In this study, Solexa deep sequencing technology was used for high-throughput analysis of miRNAs in a small RNA library isolated from four M. nipponense tissues (gill, hepatopancreas, muscle and hemocytes). In total, 9,227,356 reads were obtained, 4,293,155 of which were related to 267 unique miRNAs, including 203 conserved and 64 prawn-specific miRNAs. Furthermore, miRNA features including length distribution and end variations were characterized. Annotation of targets revealed a broad range of biological processes and signal transduction pathways regulated by M. nipponense miRNAs. In addition, 880 co-expressed and 39 specific (25 normoxia-specific and 14 hypoxia-specific) miRNAs of four combined tissues of prawns that may be involved in the response to hypoxia were confirmed using miRNA microarray analysis. Real-time quantitative PCR (qPCR) analysis of eight miRNAs in the normoxia and hypoxia groups showed good concordance between the sequencing and qPCR data. This study provides the first large-scale identification and characterization of M. nipponense miRNAs and their potential targets, and represents a foundation for further characterization of their roles in the regulation of the diversity of hypoxia processes.

2016-06-01 | E-GEOD-72417 | biostudies-arrayexpress

Identification and characterization analysis of the Oriental River Prawn (Macrobrachium nipponense) microRNA response to hypoxia using a deep sequencing approach [miRNA microarray analysis]

2016-06-01 | E-GEOD-72416 | biostudies-arrayexpress

Project description:Differential expression of microRNAs in giant freshwater prawn (Macrobrachium rosenbergii) during the infection of Vibrio parahaemolyticus

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