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ABSTRACT: Effects of dietary supplementation with cysteamine on rumen microflora in Hu lambs

PROVIDER: PRJNA1268342 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33708148_1.fastq.gz	Fastqsanger.gz
	SRR33708148_2.fastq.gz	Fastqsanger.gz
	SRR33708149_1.fastq.gz	Fastqsanger.gz
	SRR33708149_2.fastq.gz	Fastqsanger.gz
	SRR33708150_1.fastq.gz	Fastqsanger.gz

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Project description:Cistus ladanifer L. is a common shrub endemic to the Mediterranean that is highly concentrated in condensed tannins (CT). CT form complexes with dietary protein that resist microbial degradation in the rumen, which enhances dietary protein utilization in ruminant diets. The objective of this study was to evaluate the utilization of CT in the diet of lambs on the proteomes of muscle, hepatic and adipose tissues. Twenty-four white merino ram lambs were divided in three groups (n=8) fed on different diets: control (16% crude protein - CP), reduced protein (12% CP) and reduced protein treated with CT extract. At the end of the trial, lambs were slaughtered and the longissimus lumborum muscle, hepatic and peri-renal adipose tissues sampled. A two-way approach was used for shotgun proteomic analysis: 2D-DIGE (gel-based) and nanoLC-MS (gel-free). In the muscle, control lambs had lower abundance proteins that partake in the glycolysis pathway. With dietary 12 % CP, lambs had higher abundance of Fe-carrying proteins in the hepatic tissue. Lambs with dietary CT had higher abundance of hepatic flavin reductase. In the adipose tissue, control lambs had lower abundance of fatty-acid synthase. In conclusion, CT inclusion influences specific pathways in lamb tissues.

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