Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide methylation analysis showng allele-specific DNA methylation

ABSTRACT: Genome-wide methylation analysis showng allele-specific DNA methylation

PROVIDER: PRJNA1269047 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Chromosome wide analysis of parental allele specific chromatin and DNA methylation in mouse

Project description:This SuperSeries is composed of the following subset Series: GSE26720: Chromosome wide analysis of parental allele specific chromatin and DNA methylation GSE26891: Chromosome wide analysis of parental allele specific chromatin and DNA methylation along mouse distal chr7 GSE26892: Chromosome wide analysis of parental allele specific chromatin and DNA methylation along the central part of mouse chr7 GSE26893: Chromosome wide analysis of parental allele specific chromatin and DNA methylation along mouse distal chr15 Refer to individual Series

2011-03-31 | E-GEOD-26947 | biostudies-arrayexpress

Chromosome wide analysis of parental allele specific chromatin and DNA methylation along mouse distal chr15

Project description:Imprinted genes are monoallelically expressed according to parental inheritance. The maternally and paternally inherited alleles are distinguished epigenetically by DNA methylation and histone modifications. Chromosome-wide Chromatin immunoprecipitation (ChIP) and MIRA analysis of MatDup.dist7 and PatDup.dist7 MEFs provided a panoramic map of reciprocal allele-specific histone modifications and DNA methylation at imprinted genes along distal chromosome 7 and 15. ChIP-chip and MIRA-chip was done to map histone modifications and DNA methylation along distal chr15 in the maternal allele and paternal allele in Patdup.dist7=MatDup.dist15 and Patdup.dist7=MatDupdis15 MEFs, respectively, using Nimblegen tiling arrays for chr15.

2011-03-31 | E-GEOD-26893 | biostudies-arrayexpress

Chromosome wide analysis of parental allele specific chromatin and DNA methylation along mouse distal chr7

Project description:Imprinted genes are monoallelically expressed according to parental inheritance. The maternally and paternally inherited alleles are distinguished epigenetically by DNA methylation and histone modifications. Chromosome-wide Chromatin immunoprecipitation (ChIP) and MIRA analysis of MatDup.dist7 and PatDup.dist7 MEFs provided a panoramic map of reciprocal allele-specific histone modifications and DNA methylation at imprinted genes along distal chromosome 7 and 15. ChIP-chip and MIRA-chip was done to map histone modifications and DNA methylation along distal chr7 in the maternal allele and paternal allele in Matdup.dist7 and Patdup.dist7 MEFs, respectively, using Nimblegen tiling arrays for distal chr7.

2011-03-31 | E-GEOD-26891 | biostudies-arrayexpress

Chromosome wide analysis of parental allele specific chromatin and DNA methylation along mouse distal chr15

2011-03-31 | GSE26893 | GEO

Chromosome wide analysis of parental allele specific chromatin and DNA methylation along mouse distal chr7

2011-03-31 | GSE26891 | GEO

Chromosome wide analysis of parental allele specific chromatin and DNA methylation along the central part of mouse chr7

Project description:Imprinted genes are monoallelically expressed according to parental inheritance. The maternally and paternally inherited alleles are distinguished epigenetically by DNA methylation and histone modifications. Chromosome-wide Chromatin immunoprecipitation (ChIP) and MIRA analysis of MatDup.dist7 and PatDup.dist7 MEFs provided a panoramic map of reciprocal allele-specific histone modifications and DNA methylation at imprinted genes along distal chromosome 7 and 15. ChIP-chip and MIRA-chip was done to map histone modifications and DNA methylation along central chr7 in the maternal allele and paternal allele in Matdup.dist7 and Patdup.dist7 MEFs, respectively, using Nimblegen tiling arrays for central chr7.

2011-03-31 | E-GEOD-26892 | biostudies-arrayexpress

Chromosome wide analysis of parental allele specific chromatin and DNA methylation along the central part of mouse chr7

2011-03-31 | GSE26892 | GEO

Allele-specific DNA methylation in the livebearing fish Poeciliopsis gracilis

Project description:Allele-specific DNA methylation in the livebearing fish Poeciliopsis gracilis

| PRJEB46512 | ENA

Allele-specific Methylation of Human Placental Tissue

Project description:In this study, we screened human placental samples for allele-specific methylation and subsequently novel imprinted genes associated with these regions. We used reduced representation bisulfite sequencing to identify partially methylated CpG islands (CGIs) in the human placental genome. We were able to delineate potential candidates for allele-specific methylation based on the calculation of a concordance statistic. Amongst the 28 regions chosen for validation based on high levels of expression, two regions were shown to exhibit allele-specific expression. Single base-resolution methylation analysis in the placental genome and RNA-Seq

2013-10-09 | E-GEOD-40955 | biostudies-arrayexpress

Genome-wide methylation analysis showng allele-specific DNA methylation

Project description:Prader-Willi Syndrome (PWS) is caused by loss of expression of paternally expressed genes in the human 15q11.2-q13 imprinting domain. A set of imprinted genes that are active on the paternal but silenced on the maternal chromosome are intricately regulated by a bipartite imprinting center (PWS-IC) located in the PWS imprinting domain. We previously discovered that euchromatic histone lysine N-methyltransferase-2 (EHMT2/G9a) inhibitors are capable of un-silencing PWS-associated genes by restoring their expression from the maternal chromosome. Here, in mice lacking the Ehmt2 gene, we document un-silencing of the imprinted Snrpn/Snhg14 gene on the maternal chromosome in the late embryonic and postnatal brain. Using PWS and Angelman syndrome patient derived cells with either paternal or maternal deletion of 15q11.2-q13, we have found that chromatin of maternal PWS-IC is closed and has compact 3D folding confirmation. We further show that a distinct noncoding RNA (TSS4-280118) preferentially transcribed from the upstream of the PWS-IC of maternal chromosome interacts with EHMT2 and forms a heterochromatin complex in CIS on the maternal chromosome. Inactivation of TSS4-280118 by CRISPR/Cas9 editing results in unsilencing of the expression of SNRPN and SNORD116 from the maternal chromosome. Taken together, these findings demonstrate that allele-specific recruitment of EHMT2 is required to maintain the maternal imprints. Our findings provide mechanistic insights and support a model for imprinting maintenance of the PWS imprinted domain.

2025-05-28 | GSE298378 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data