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Transcriptomic signatures associated with regenerative wound vs. fibrotic repair outcomes to lens post-cataract surgery wounding

ABSTRACT: Transcriptomic signatures associated with regenerative wound vs. fibrotic repair outcomes to lens post-cataract surgery wounding

PROVIDER: PRJNA1269530 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33756259_1.fastq.gz	Fastqsanger.gz
	SRR33756259_2.fastq.gz	Fastqsanger.gz
	SRR33756260_1.fastq.gz	Fastqsanger.gz
	SRR33756260_2.fastq.gz	Fastqsanger.gz
	SRR33756261_1.fastq.gz	Fastqsanger.gz

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Transcriptomic signatures associated with regenerative wound vs. fibrotic repair outcomes to lens post-cataract surgery wounding

Project description:RNAseq analysis was used to determine the transcriptomic changes associated with regenerative vs. fibrotic repair to lens injury. Using an ex vivo post-cataract surgery model, we can follow two distinct outcomes to lens injury: 1) A regenerative wound healing response that ensues across the cell-denuded lens capsule basement membrane (BM), culminating in wound closure on Day 3 (D3) post-injury. 2) Induction of fibrosis, which occurs when a wound healing response occurs off the lens capsule, termed the extracapsular zone (ECZ), where transition to a fibrotic phenotype occurs on day 3 post-injury. Here, we report the changes in the transcriptome that occur on D1, D2, and D3 post-injury, a period that covers wound healing across the BM and time points prior to (D1, D2) and after induction of the fibrotic phenotype (D3). Our data provides a molecular map for understanding the gene changes associated with navigating normal and pathological outcomes to lens injury.

2025-06-02 | GSE298481 | GEO

Changes in gene expression time 0 vs. 1hr post-cataract sugery wounding in the lens

Project description:Early changes in the transcriptome associated with lens wounding in an ex vivo post-cataract surgery chicken model. Here we report the changes in the transcriptome that occur 1hr vs. time 0 post-cataract surgery wounding. Our data provide a molecular framework for understanding the early gene changes associated with the injury response of the lens.

2023-11-04 | GSE239368 | GEO

RNA-seq Analysis of Human Lens Capsules from Donors with and without Cataract Surgery History

Project description:Cataract surgery is the most commonly performed ophthalmic procedure worldwide, yet the long-term molecular adaptations of lens epithelial cells (LECs) following surgery remain poorly understood. In this study, we conducted bulk RNA sequencing on lens capsules from 34 human donors, including 17 with an average of 7 years post-cataract surgery and 17 non-cataract controls. Differential expression analysis revealed substantial transcriptional changes in post-surgical LECs, including 1,690 upregulated and 579 downregulated genes. Gene Set Enrichment Analysis (GSEA) and Gene Ontology (GO) analyses identified activation of oxidative stress response, cell survival pathways, and immune response signatures. Collectively, our findings reveal that LECs undergo profound and sustained molecular reprogramming following cataract surgery, including inflammatory, fibrotic, migratory, and senescent changes. These adaptations may contribute to posterior capsule opacification (PCO) and highlight potential therapeutic targets for its prevention.

2025-07-23 | GSE295383 | GEO

Smurf1 modulates Smad signaling pathway in fibrotic cataract formation [mouse_ASC]

Project description:Purpose: TGF-β/BMP signaling pathway has a significant role in fibrotic cataract. Smurf1, a ubiquitin protein ligase, regulates the TGF-β/BMP signaling pathway through the ubiquitin-proteasome system (UPS). This study aims to investigate the role of Smurf1 in the progression of fibrotic cataract and its underlying mechanism.Method: We employed a mouse injury-induced anterior subcapsular cataract (ASC) model and administered Smurf1 inhibitor A01 through anterior chamber injection for in vivo investigation. RNA sequencing was performed to examine global gene expression changes. The volume of the subcapsular opacity was determined using whole-mount immunofluorescence of lens anterior capsules. Lentivirus was utilized to create cell lines with Smurf1 knockdown or overexpression in SRA01/04. Protein levels were assessed by Simple Western. Lens epithelial cell (LEC) proliferation was evaluated by CCK8 and EdU assays. LEC migration was measured by Transwell and wound healing assays.Results: The mRNA levels of genes associated with cell proliferation, migration, epithelial-mesenchymal transition (EMT), TGF-β/BMP pathway and UPS, including Smurf1, were upregulated in ASC model. The mRNA expression of Smurf1 was also upregulated in anterior lens capsules of age-related cataract patients. Anterior chamber injection of A01 inhibited ASC formation and EMT. In vitro knockdown of Smurf1 resulted in reduced proliferation, TGF-β2-induced migration and EMT of LECs. Smurf1 inhibition upregulated Smad1, Smad5 and pSmad1/5. Conversely, Smurf1 overexpression displayed the opposite phenotypes.Conclusion: Smurf1 regulated the progression of fibrotic cataract by influencing the proliferation, migration and EMT of LECs through regulation of Smad signaling pathway, offering a novel target for the treatment of fibrotic cataract.

2024-02-14 | GSE245411 | GEO

Lens epithelial cell transcriptome reprogramming at 24 hours post cataract surgery

Project description:This trancriptome analysis reveals the first detailed analysis on how the remnant lens epithelial cells (LECs) greatly reprogram their transcriptome towards inflammation/fibrosis by 24 hours post cataract surgery (PCS).

2018-10-18 | GSE111430 | GEO

Transcriptomic changes in lens epithelial cells at 72 hours post cataract surgery

Project description:This trancriptomic analysis describes the gene expression changes observed in wildtype lens epithelial cells (LECs) by 72 hours compared to zero hours post cataract surgery

2025-08-05 | GSE267060 | GEO

Transcriptomic changes in lens epithelial cells at 5 days post cataract surgery

Project description:This trancriptomic analysis describes the gene expression changes observed in wildtype lens epithelial cells (LECs) by 5 days compared to zero hours post cataract surgery

2025-08-05 | GSE266635 | GEO

Smurf1 modulates Smad signaling pathway in fibrotic cataract formation [cell line]

2024-02-14 | GSE245407 | GEO

Expression data from lens epithelial cells (LECs) from Shumiya cataract rats (SCR) with or without cataract (Cat+ or Cat-)

Project description:The Shumiya cataract rat (SCR) is a model for hereditary cataract. Two-third of these rats develop lens opacity within 10-11-weeks. Onset of cataract is attributed to the synergetic effect of lanosterol synthase (Lss) and farnesyl-diphosphate farnesyltransferase 1 (Fdft1) mutant alleles that lead to cholesterol deficiency in the lenses, which in turn adversely affects lens biology including the growth and differentiation of lens epithelial cells (LECs). Nevertheless, the molecular events and changes in gene expression associated with the onset of lens opacity in SCR is poorly understood. Our study aimed to identify the gene expression patterns during cataract formation in SCRs, which may be responsible for cataractogenesis in SCR.

2020-07-31 | GSE152616 | GEO

Changes in gene expression time 0 vs. 1hr post-cataract sugery wounding in the lens

Project description:Changes in gene expression time 0 vs. 1hr post-cataract sugery wounding in the lens

| PRJNA998907 | ENA