Project description:Magnaporthe oryzae (rice blast) and the root-knot nematode Meloidogyne graminicola are causing two of the most important pathogenic diseases jeopardizing rice production. Here, we show that root-knot nematode infestation on rice roots leads to important above-ground changes in plant immunity gene expression, which is correlated with significantly enhanced susceptibility to blast disease.
Project description:The aim of this study was to elucidate the molecular basis underlying the compatible interaction between potato and root-knot nematode at early stages on infection at 3 and 7dpi.
Project description:In this experiment we sequence the whole genome DNA of 28 unique populations of the root-knot nematode Meloidogyne hapla to investigate their genetic diversity using illumina sequencing. The hapla populations came from various locations, mostly in the Netherlands and Europe. The nematodes were maintained on tomato cv. Money Maker in the greenhouse of Wageningen University. After DNA extraction, DNA was sent for sequencing. The resulting sequence was used for calling variants and genetic analysis.
Project description:High-coverage whole genome sequencing of 11 Brazilian isolates of the root-knot nematode Meloidogyne incognita, presenting different host plant preferences and different geographical origins. Four M. incognita host races had been proposed in the past, based on host (in)compatibility on four different plant strains. The objective was to assess whether genomic variations (SNP) correlate with host range compatibility, geographical origin and host plant of origin.
Project description:Transcriptome profiling of roots from Oryza sativa ssp. japonica cv. Nipponbare and Nipponbare mutant in response to root-knot nematode (RKN, Meloidogyne graminicola) infection. The experiment aimed to identify differentially expressed genes during early nematode establishment. Roots were collected at three time points: 0 days (uninfected control), 3 days, and 7 days post-inoculation. Each condition had three biological replicates. Total RNA was extracted and rRNA-depleted prior to library construction and sequencing.
Project description:We compared the gene expression of wild-type Col-0 and a T-DNA mutant SALK_116381C (opr2-1). We either infected or mock-infected the plants with the root knot nematode Meloidogyne incognita and measured the root transcriptome after 0, 1, 4, and 7 days post infection using RNA-seq. The aim of the experiment was to determine whether opr2-1 affected gene expression patterns induced by nematode infection.
