Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The Genomic and Transcriptomic Landscape of a HeLa Cell Line

ABSTRACT: The Genomic and Transcriptomic Landscape of a HeLa Cell Line

PROVIDER: PRJNA1273210 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

CNOT1 and Transcriptomic Landscape of a HeLa Cell Line

Project description:CNOT1 and Transcriptomic Landscape of a HeLa Cell Line

| PRJNA593610 | ENA

The genomic and transcriptomic landscape of a HeLa cell line.

Project description:HeLa is the most widely used model cell line for studying human cellular and molecular biology. To date, no genomic reference for this cell line has been released, and experiments have relied on the human reference genome. Effective design and interpretation of molecular genetic studies performed using HeLa cells require accurate genomic information. Here we present a detailed genomic and transcriptomic characterization of a HeLa cell line. We performed DNA and RNA sequencing of a HeLa Kyoto cell line and analyzed its mutational portfolio and gene expression profile. Segmentation of the genome according to copy number revealed a remarkably high level of aneuploidy and numerous large structural variants at unprecedented resolution. Some of the extensive genomic rearrangements are indicative of catastrophic chromosome shattering, known as chromothripsis. Our analysis of the HeLa gene expression profile revealed that several pathways, including cell cycle and DNA repair, exhibit significantly different expression patterns from those in normal human tissues. Our results provide the first detailed account of genomic variants in the HeLa genome, yielding insight into their impact on gene expression and cellular function as well as their origins. This study underscores the importance of accounting for the strikingly aberrant characteristics of HeLa cells when designing and interpreting experiments, and has implications for the use of HeLa as a model of human biology.

| S-EPMC3737162 | biostudies-literature

The genomic Landscape of an HeLa cell line.

Project description:Genotyping by high-throughput sequencing of HeLa Kyoto H2B-mRFP/mEGFP-alpha-tubulin cell line

| PRJEB426 | ENA

CNOT1 and Transcriptomic Landscape of a HeLa Cell Line

Project description:Purpose: The goals of this study are to compare NGS-derived total transcriptome profiling (RNA-seq) of CNOT1-depleted cells (using siRNA) with control HeLa cells before and after DNA damage to examine quantitative gene expression.

2019-12-05 | GSE141496 | GEO

Genomic landscape of meningiomas

Project description:This SuperSeries is composed of the following subset Series: GSE16581: Genomic landscape of meningiomas: gene expression GSE16583: Genomic landscape of meningiomas: genotyping Refer to individual Series

2010-12-01 | E-GEOD-16584 | biostudies-arrayexpress

Comparative genomic hybridization of human genomic DNA and nucleolus-associated DNA in HeLa cell line.

Project description:The aim of the experiment was to identify nucleolus-associated chromosomal domains. HeLa exp samples represent DNA from isolated nucleoli, whereas HeLa ref samples total genomic DNA of HeLa cells. "exp" samples were Cy5 labeled and hybridized together with Cy3-labeled "ref" samples on a Nimblegen 385K WG CGH platform. The DNA samples were not amplified.

2010-10-11 | E-MEXP-2403 | biostudies-arrayexpress

Brassica napus RCC-S0 (synthetic line), genomic and transcriptomic data.

Project description:Brassica napus RCC-S0 (synthetic line), genomic and transcriptomic data.

| PRJEB71516 | ENA

HeLa cell line exhibits single-clonal growth variation

Project description:The HeLa cell line is the first human-derived immortalized cell line， one of the most widely used cell lines in the world. While many researches had pointed out the heterogeneity of HeLa cells across laboratories and the low repeatability of experiments performed on HeLa cells, the reason how these heterogeneities generated is little known. We sorted cells into single cells, cultured them to single clones and performed multi-omics sequencing. The single cell-derived clones exhibited persistent growth variation both in vitro and in vivo. The corresponding WGS analysis revealed the single clones diverse in copy number even at the whole chromosome level. Our work explored the intrinsic mechanisms of the low repeatability of cell line experiments. We also provided a resource for single cell-derived clones.

2025-08-25 | GSE181890 | GEO

Homo sapiens

Project description:The Genomic and Transcriptomic Landscape of a HeLa Cell Line

| PRJNA214225 | ENA

EWS siRNA knock-down in HeLa cell line

Project description:HeLa cell line was trasfected by a EWS siRNA oligo causing knock-down of EWS or a siRNA scrambled oligo

2011-09-20 | GSE22929 | GEO