Dataset Information

ERAP1 deficiency exacerbates DSS-induced colitis and alters sulfasalazine efficacy via immune dysregulation

ABSTRACT: ERAP1 deficiency exacerbates DSS-induced colitis and alters sulfasalazine efficacy via immune dysregulation

PROVIDER: PRJNA1275756 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	SRR33949973.fastq.gz	Fastqsanger.gz
	SRR33949974.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Project description:Ulcerative colitis (UC) is a life-threatening heterogeneous condition characterized by inflammation of the colon. Endoplasmic reticulum aminopeptidase 1 (ERAP1) is essential for antigen processing and immune regulation, however, its specific role in UC pathogenesis and therapeutic response remains unclear. This study aimed to investigate the role of ERAP1 in the response to sulfasalazine, a standard treatment for UC, using an ERAP1-heterozygous (ERAP1⁺/⁻) mouse model susceptible to colitis. Wild-type (WT) and ERAP1+/− mice were treated with 2.5% dextran sulfate sodium (DSS) to induce colitis, followed by sulfasalazine administration. Colitis severity was assessed through histopathology. Immune cell populations, including neutrophils, dendritic cells, T cells, and NK1.1+ cells, were analyzed using flow cytometry. RNA sequencing of colonic tissues was performed to assess gene expression changes associated with reduced ERAP1 expression. ERAP1+/− mice exhibited more severe DSS-induced colitis, with greater weight loss and increased mucosal damage compared to WT mice. In addition, ERAP1+/− colitis mice showed marked changes in peripheral blood and intestinal intraepithelial lymphocyte (IELs) immune cell infiltration after sulfasalazine treatment. RNA sequencing identified 428 differentially expressed genes between ERAP1⁺/⁻ and WT mice. Among these, 28 genes were previously associated with colitis or colorectal cancer, of which 11 were upregulated and 17 downregulated in ERAP1⁺/⁻ mice. RT-qPCR analysis further confirmed that the expression of Anxa9, Atp2a1, and Hepacam2 was significantly elevated in ERAP1⁺/⁻ mice following sulfasalazine treatment, suggesting a differential therapeutic response. Collectively, our study demonstrates that partial ERAP1 deficiency exacerbates DSS-induced colitis by promoting immune dysregulation and altering the expression of inflammation-related genes. These findings suggest that reduced ERAP1 expression may impair the therapeutic efficacy of sulfasalazine. Therefore, ERAP1 may serve as a key regulator in the pathogenesis of UC and a potential target for therapy.

Dataset Information

ERAP1 deficiency exacerbates DSS-induced colitis and alters sulfasalazine efficacy via immune dysregulation

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets