Project description:The mutualistic arbuscular mycorrhizal (AM) symbiosis arose in land plants more than 450 million years ago. This symbiosis is still widely found across major land plant lineages, including bryophytes. Despite its broad taxonomic distribution, little is known about the molecular components underpinning symbiosis outside of flowering plants. Here, we demonstrate that a broad AM genetic programme is conserved amongst land plants. In this study, we characterised the dynamic response of the liverwort Marchantia paleacea to Rhizophagus irregularis colonization by time-resolved transcriptomics across three stages of symbiosis. Comparative analysis of transcriptional responses to symbiosis in the liverwort M. paleacea and the legume Medicago truncatula further revealed evolutionarily conserved expression patterns for genes underpinning pre-symbiotic signalling, intracellular colonization and nutrient exchange. This study demonstrates that the genetic machinery regulating key aspects of symbiosis in plant hosts is largely conserved and coregulated across land plants.
Project description:To find the downstream signaling components upon CO4 or CO7 perception, we performed proteomics and phosphoproteomics analysis with M. paleacea treated with 10-5 M CO4 or CO7 for 10 mins. For the proteomics and phosphoproteomics analysis, 14-day-old gemmalings of M. paleacea growing on 1/2 Gamborg’s B5 medium (pH 5.8) containing 1% agar were sampled and floated on 2 ml sterilized water in a 6-well plate, and then incubated overnight. The water was replaced with the solution containing 10-5 M CO4, 10-5 M CO7, or mock for 10 min. The system consisted of a timsTOF Pro2 mass spectrometer (Bruker Daltonics) equipped with a nano elute system was used for proteomics and phosphoproteomics analysis.