Project description:Quinoa is widely recognized for its exceptional nutritional properties, particularly its complete protein content. This study, for the first time, investigates the effects of processing methods (boiling and extrusion) and farming conditions (conventional and organic) on the quinoa’s proteomic profile. Following a label-free shotgun proteomics approach, a total of 1,796 proteins were identified and quantified across all quinoa samples. Regarding processing, both boiling and extrusion produced protein extracts with lower total protein content, with the number of identified proteins decreasing from 1,695 in raw quinoa to 957 in processed quinoa. Boiling led to a reduction in protein diversity and expression, while extrusion, which involves high temperatures and pressures, specifically decreased the abundance of high molecular mass proteins. Concerning cultivation practices, organic farming was associated with a broader protein diversity, especially proteins related to translation (28% vs. 5%), while conventional farming showed a higher abundance of catalytic and enzymatic proteins (67% vs. 46%). These findings highlight the distinct proteomic changes induced by different processing methods and farming conditions, offering valuable insights to manage quinoa’s nutritional, bioactive, and functional properties across various production practices.
2025-01-17 | PXD050043 | Pride
Project description:Long-term Organic farming in Quzhou
| PRJNA388750 | ENA
Project description:Soil microbiome from organic and conventional farming
| PRJNA1344597 | ENA
Project description:Network project on Organic Farming - Fungal Diversity
Project description:DNA methylation is an epigenetic mark that can be transmitted from one generation to the next. DNA methylation patterns can be specific to environmental conditions. In our ap-proach, differences in DNA methylation pattern were compared among tubers of potato (Solanum tuberosum) grown under organic and conventional farming conditions. These conditions differed in application of fertilizer, herbicides, fungicides and insecticides. Samples grown at two different years under organic and conventional growing conditions in three independent field replicates were analyzed to identify differentially methylated regions (DMRs). Post-bisulfite-adapter tagging whole-genome bisulfite-sequencing (PBAT-WGBS) was performed on the extracted DNA. Only using relaxed selection param-eters revealed sixty shared DMRs were identified among both years analyzed. One of the identified DMR was associated with the StATOX1 gene. StATOX1 is an evolutionarily highly conserved antioxidant copper chaperone, responsible for detoxification of copper in organisms. Copper content of the potato samples was measured by Inductively-Coupled Plasma-Atomic-Emission-Spectrometry (ICP-AES) after high pressure nitric acid oxidation. As organic potato management relies on the application of copper as fungicide, our iden-tified region might be indicative for the application of this specific compound.