Project description:Purpose: Determine the mechanism of particulate matter-induced signaling in melanocytes. Method: Primary human epidermal melanocytes were treated with particulate matter (5 μg/cm2) and incubated for 24 h. Total RNA (1 ug) from melanocytes were extracted and subjected to library synthesis. Results: Particulate matter-treated melanocytes exhibited upregulation of ER stress, unfolded protein response, and melanogenesis-related molecules. Conclusion: Particulate matter-induced melanocyte signaling was well evaluated using RNA sequencing.
Project description:Fine particulate matter (PM2.5) is an environmental pollutant associated with adverse health effects, including increased cancer risk. To investigate early transcriptional responses to PM2.5 exposure in non-malignant epithelial cells, we performed bulk RNA sequencing of wildtype mouse mammary epithelial EpH4 cells cultured under two-dimensional (2D) conditions. Cells were either left untreated or exposed to PM2.5. This dataset captures genome-wide gene expression changes induced by PM2.5 in wildtype epithelial cells and provides insight into stress response and pathophysiological pathways activated upon particulate matter exposure.
2026-07-01 | GSE336715 | GEO
Project description:ITS2 rRNA sequencing of Acropora formosa
| PRJNA1444880 | ENA
Project description:Acropora formosa-symbiotic Symbiodiniaceae composition under high-temperature/low-temperature stress
Project description:Here we used next generation sequencing (NGS), to determine the transcriptional profile of blood cells exposed to particulate matter to contribute to the clarification of the importance of deregulated molecules in the molecular pathways involved in the inflammation. For this, blood cells from six adult healthy donors were treated with particulate matter.
