Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA127989 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:HGF-CM increases the proliferation and migration ability of DPSC (Dental pulp stem cells) in a dosage dependent manner, and facilitates the mineralization of DPSC by upregulating odotogenic genes. Although lack of in vitro evidence, DPSC and hGF-CM could be a promising combination for pulp regeneration in the future.
2022-10-29 | GSE201524 | GEO
Project description:Stem cell-based therapy is an alternative strategy for brain repair. Various cell types have been investigated and dental pulp stem cells (DPSC) have been identified as promising candidates. These multipotent stem cells are found in the dental pulp tissue of molar teeth. They are clinically easily obtained, have a high proliferative rate, and possess neurogenic potential due to their mesoectodermal origin. Here, overexpression of octamer-binding transcription factor 4 (OCT4) in combination with neural conditions was used to reprogram human DPSC along the neural lineage. Transcriptomic analysis of differentially-expressed genes highlighted the expression of genes associated with neural and neuronal functions in the OCT4-overexpressing DPSC following neural induction.
2024-03-06 | GSE237184 | GEO
Project description:A major challenge to the study and treatment of neurogenetic syndromes is the difficulty in gaining access to live neurons from individuals with these disorders. Although other sources of stem cells are currently available for differentiation into neurons, these can involve invasive procedures and be difficult or expensive to generate limiting their use on a broad scale, especially for rare syndromes which may not be well represented in the local population. Dental pulp stem cells (DPSC) are neural crest derived multipotent stem cells that reside deep the pulp of shed (baby) teeth and have the potential for broad use in the study of neurogenetic disease. In order to investigate the characteristics of DPSC which make them a valuable resource for the study of neurogenetic syndromes we performed a set of viability, senescence and immortalization studies on control DPSC and DPSC derived neurons. We investigated the basic transport conditions and determined the maximum passage number for primary DPSCs. We then immortalized control DPSC using human telomerase reverse trancriptase (hTERT) and evaluated both neuronal differentiation potential and gene expression changes using RNAseq. Here we show that immortalized DPSC share morphological and electrophysiological properties with non-immortalized DPSC. We also show that differentiation of DPSC into neurons changes gene expression for 1305 transcripts, while immortalized neurons differ significantly in gene expression for 183 transcripts of which 94 also changed during differentiation. Taken together, these studies indicate that immortalized dental pulp derived neruons may be a new and powerful resource for the study of rare neurological disorders where patient samples are rare or difficult to obtain. RNA-seq Analysis of 3 neurotypical control DPSC, 3 DPSC derived neurons and 3 each immortalized versions of DPSC and DPSC neurons (~3 weeks post maturation)
2016-08-08 | E-GEOD-67124 | biostudies-arrayexpress
Project description:Neoplastic transformation of DPSC cultured under Hypoxia versus normoxia. Molecular characterization of cell markers associated with tumorigenicity. DPSC array CGH profiles of experimental (HX48h and HX72h) and reference (NX48 and NX72h) genomic DNA samples
2013-07-01 | E-GEOD-22673 | biostudies-arrayexpress
Project description:Neoplastic transformation of DPSC cultured under Hypoxia versus normoxia. Molecular characterization of cell markers associated with tumorigenicity.
2013-07-01 | GSE22673 | GEO
Project description:To evaluate the miRNA and mRNA expression profiles (miRNOME) we identified miRNAs during in vitro osteogenic differentiation of human dental pulp stem cells (DPSC). The DPSCs were cultured in the DMEM + beta-glycerol phosphate, ascorbic acid and dexamethasone for 2 dias to 21days. The microRNA or mRNA expression profiling during the differentiation process was analyzed through hybridizations with Agilent miRNA-microarray (8x15K format).
2014-11-28 | E-MTAB-3077 | biostudies-arrayexpress
Project description:A major challenge to the study and treatment of neurogenetic syndromes is the difficulty in gaining access to live neurons from individuals with these disorders. Although other sources of stem cells are currently available for differentiation into neurons, these can involve invasive procedures and be difficult or expensive to generate limiting their use on a broad scale, especially for rare syndromes which may not be well represented in the local population. Dental pulp stem cells (DPSC) are neural crest derived multipotent stem cells that reside deep the pulp of shed (baby) teeth and have the potential for broad use in the study of neurogenetic disease. In order to investigate the characteristics of DPSC which make them a valuable resource for the study of neurogenetic syndromes we performed a set of viability, senescence and immortalization studies on control DPSC and DPSC derived neurons. We investigated the basic transport conditions and determined the maximum passage number for primary DPSCs. We then immortalized control DPSC using human telomerase reverse trancriptase (hTERT) and evaluated both neuronal differentiation potential and gene expression changes using RNAseq. Here we show that immortalized DPSC share morphological and electrophysiological properties with non-immortalized DPSC. We also show that differentiation of DPSC into neurons changes gene expression for 1305 transcripts, while immortalized neurons differ significantly in gene expression for 183 transcripts of which 94 also changed during differentiation. Taken together, these studies indicate that immortalized dental pulp derived neruons may be a new and powerful resource for the study of rare neurological disorders where patient samples are rare or difficult to obtain.
2016-08-08 | GSE67124 | GEO
Project description:To evaluate the miRNA and mRNA expression profiles (miRNOME and transcriptome) we reconstructed networks identifying miRNAs and mRNA during in vitro osteogenic differentiation of human dental pulp stem cells (DPSC). The DPSCs were cultured in the DMEM + beta-glycerol phosphate, ascorbic acid and dexamethasone for 2 to 21 days. The microRNA or mRNA expression profiling during the differentiation process was analyzed through hybridizations with Agilent miRNA-microarray (8x15K format) or whole-human genome Agilent microarray (4x44K format).
2014-11-13 | E-MTAB-3010 | biostudies-arrayexpress
Project description:Teeth are a significant source of stem cells and have clinical importance for regenerative medicine. A human tooth harbors different kinds of stem cells in the dental pulp (DPSC) or the periodontal ligament (PDLSC). Also exfoliated teeth in childhood contain a special type of stem cells in their pulp called Stem cells from Human Exfoliated Deciduous teeth (SHED). All these stem cells have features and capacities that vary depending on their niche. Evaluating dental-originated stem cells via molecular analysis tools is important to gain insight into their regenerative potential. Here we investigated the proteomic properties of three types of stem cells that originated from human teeth. We isolated and cultured the DPSCs, PDLSCs, and SHED cells. After validating MSC populations via immunophenotyping, we performed a mass spectrometry-based proteomic approach to identify and relatively quantify whole cell and secreted proteins. Identified proteins were evaluated by using Gene Ontology and Reactome pathway analysis tools. Our data reveal that SHED cells represented inflammation, hypoxia, and nutrient deficiency-associated ontologies in both their secretome and whole-cell proteomes. The whole-cell proteome of PDLSCs consisted of differentiation and proliferation-associated molecules while their secretory molecules were mainly associated with inflammation, ECM organization, and immune response ontologies. Among dental-originated stem cells, DPSCs appeared to be the healthiest and clinically relevant in terms of proteomic properties with their proliferation, growth factor signaling, and stemness-associated pathways and ontologies in their secretome and whole-cell proteome. Obtained results demonstrated that every type of stem cell from dental origin has unique proteomic features that are altered by their location and physiological conditions. Considering these findings may help researchers improve the dental stem-cell-based regenerative medicine approaches.
2026-04-07 | PXD058096 | Pride
Project description:Dental pulp stem cells (DPSC) constitute a neural crest-derived stem cell population endowed with multipotency and self-renewal. While the process of DPSC differentiation has been studied extensively in vitro, very little is known about mechanisms underpinning the differentiation of human DPSC in vivo. Here, we induced vasculogenic, odontoblastic, or neurogenic differentiation of human DPSC for 7 days in vitro and performed single cell sequencing. Then, human DPSC tagged with GFP (DPSC-GFP) seeded in human tooth slice/scaffolds were transplanted into immunodeficient mice. Single cell sequencing of DPSC-GFP sorted by flow cytometry was performed 7 and 21 days after transplantation. We observed major shifts in patterns of gene expression when DPSC were induced to undergo vasculogenic, odontoblastic, or neurogenic differentiation in vitro. Although some DPSC retained mesenchymal stem cell (MSC) markers (indicating asymmetric cell division and self-renewal), each differentiation protocol resulted in a unique gene expression signature in vitro. In vivo, stem cell markers that were highly expressed in DPSC pre-transplantation gradually decreased in expression after 7 and 21 days. In contrast, vascular endothelial cell markers were highly expressed 7 days after transplantation, while neuronal markers were highly expressed 21 days after transplantation. In conclusion, DPSC cells are heterogeneous with clearly distinct cell clusters, all of which contain cells with unique differentiation potential. Notably, the microenvironment created when human DPSC are transplanted inside a human root canal in vivo induces vasculogenic differentiation first, which is then followed by neurogenic differentiation.
2025-08-05 | GSE288770 | GEO