Project description:This study involves characterization of four head and neck cancer cell lines -- NT8e, OT9, AW13516 and AW8507, established from Indian head and neck cancer patients, using SNP arrays, whole exome and whole transcriptome sequencing.
Project description:Aim: investigate how the Wnt-driven Mll1 epigenome regulates salivary gland and head and neck cancer. We performed mRNA-seq and ChIP-seq of H3K4me1, me2 and me3 on mouse salivary gland cancer cells that are kept in two different growth conditions, adherent culture and non-adherent sphere culture. Mouse salivary gland cancer cells were isolated from salivary gland of transgenic mouse that harbor K14-Cre-induced Wnt/β-catenin gain-of-function and Bmpr1a loss-of-function mutations.
Project description:Ionizing radiation (IR) – induced salivary gland damage is a common adverse effect in radiotherapy for patients with head and neck cancers. Currently, there is no effective treatment for the resulting salivary gland hypofunction and xerostomia (dry mouth). Here we profiled the acute gene expression change in the mouse submandibular salivary gland, and defined its damage response patterns at the transcriptome level.
Project description:Damage to the major salivary glands is the leading long-term side effect to radiation therapy in patients with head and neck cancerof radiation of head and neck cancers,. It resultresultsing in severe hyposalivationdry mouth , which profoundly impairs basic physiological functions for patients such as speaking, eating, tasting, and swallowing. TodayCurrently, no effective treatment can be offeredexists to alleviate radiation-induced hyposalivation, but recently, treatment with adipose tissue-derived mesenchymal stem/stromal cells (ASC) hasve very recently been tested as a newshowed potential treatment modalityfor restoring the function of the salivary glands. However, the impact of ASC-treatments on the salivary gland proteinome composition has notever been testedinvestigated. HereIn this study, we examine the salivary gland proteomeuse in patients with severe hyposalivation due to radiation therapy and in healthy controls using quantitative metaproteomics. Furthermore, to 1: compare the salivary proteome in patients with severe hyposalivation to that of healthy controls, and 2: towe addresslongitudinally monitorinvestigate the effect of ASC ASC-treatment on the salivary gland proteome. We identify that treatment with ASC-treatments results inis associated with the upregulation of specific proteins in saliva 120 days after injection. Therefore, oOur data findings suggest that ASCs to stimulate biological processes in the salivary gland tissue, which could have beneficial clinical effects.
Project description:Investigating how the Wnt-driven Mll1 epigenome regulates salivary gland and head and neck cancer. We performed mRNA-seq and ChIP-seq of H3K4me1, me2 and me3 on mouse salivary gland cancer cells that are kept in two different growth conditions, adherent culture and non-adherent sphere culture. Mouse salivary gland cancer cells were isolated from salivary gland of transgenic mouse that harbor K14-Cre-induced Wnt/β-catenin gain-of-function and Bmpr1a loss-of-function mutations. Anti-H3K4me1 (C15410194), -me2 (C15410035) and -me3 (C15410003-50) antibodies were purchased from Diagenode. ChIP-seq was performed according to the protocols provided by Diagenode using the iDeal ChIP-seq kit for histones and the iDeal library preparation kit. For mRNA-seq, mRNA was extracted according to the standard TRIzol protocol (Invitrogen) and subjected to library preparation using the TruSeq stranded mRNA library preparation kit. Sequencing was performed with the TruSeq SBS Kit v3-HS (2 X 200 cycles) on an Illumina HiSeq 2000 sequencer.
Project description:We performed gene expression profiling of 39 head and neck cancer cell lines and 1 hela cell line, and classified them based on previous classification of head and neck squamous cell tumors from patients We performed gene expression profiling of 39 head and neck cancer cell lines and 1 hela cell line.
Project description:This study involves characterization of four head and neck cancer cell lines -- NT8e, OT9, AW13516 and AW8507, established from Indian head and neck cancer patients, using SNP arrays, whole exome and whole transcriptome sequencing.
Project description:This study involves characterization of four head and neck cancer cell lines -- NT8e, OT9, AW13516 and AW8507, established from Indian head and neck cancer patients, using SNP arrays, whole exome and whole transcriptome sequencing.
