Project description:A genome-scale metabolic model for the gram-positive, and polysaccharide degrading bacterium Ruminiclostridium cellulolyticum H10. The model includes manually reconstructed pathways for degradation of oligosaccharides from cellulose, xyloglucan, and arabinoxylan. The model has been compared and curated to experimental data. The draft model was initially created using CarveMe (version 1.5.1) with the gram-positive universal model as the template.
Project description:In order to improve the utilization rate of straw, Cellomonas Iranensis (CE) , a cellulose-degrading bacterium, was isolated from the gut of termites by Congo red screening and filter paper degradation re-screening. The enzyme activities of endogenous glucosidase, exogenous glucosidase and β-glucosidase were determined at the levels of 0.097 u/mL, 0.316 u/mL and 0.110 u/mL. CE was inoculated into liquid medium with filter paper or glucose as the sole carbon source and fermented for 14 days. Further transcriptomic and proteomic analyses revealed that rice straw-cultured CE was characterized by gene upregulation and gene downregulation compared with glucose-cultured CE.
