Project description:This project presents LC-MS/MS-based proteomic datasets obtained from bacterial colonies isolated from retail chicken meat samples, as part of a study focused on the detection of Campylobacter jejuni. The isolates were initially screened using a novel chromogenic enzymatic assay targeting hippuricase activity, which is specific for C. jejuni. Selected samples, including both enzymatically positive and negative results, were subjected to in-gel digestion followed by high-resolution LC-MS/MS analysis on an Orbitrap Exploris 240 instrument.
2025-09-02 | PXD063891 | Pride
Project description:Antimicrobial resistance of Salmonella isolates recovered from retail chicken in UAE
| PRJNA1062789 | ENA
Project description:Characterization of antimicrobial resistance in indicator and pathogenic bacteria from retail meat in Wyoming
| PRJNA939729 | ENA
Project description:Genomic diversity, antibiotic resistance and virulence of Listeria monocytogenes from retail meat and meat processing
Project description:A collection of 61 Salmonella enterica serovar Typhimurium (S. Typhimurium) of animal and human origin, matched as closely as possible by phage type, antimicrobial resistance pattern and place / time of isolation, and sourced from farms or hospitals in Scotland, were analysed by antimicrobial susceptibility testing, phage typing, pulsed field gel electrophoresis (PFGE), plasmid profiling and DNA microarrays. PFGE of all 61 isolates revealed ten PFGE profiles, which clustered by phage type and antibiotic resistance pattern, with human and animal isolates distributed between PFGE profiles. Analysis of 23 representative S. Typhimurium strains hybridised to a composite Salmonella DNA microarray identified a small number of specific regions of genome variation between different phage types and PFGE profiles. These variable regions of DNA were typically located within prophage-like elements. Simple PCR assays were subsequently designed to discriminate between different isolates from the same geographical region.
Project description:Persistence of Listeria monocytogenes in retail deli environments is a serious food safety issue, potentially leading to cross-contamination of ready-to-eat foods such as deli meats, salads, and cheeses. We previously discovered strong evidence of L. monocytogenes persistence in delis across multiple states. We hypothesized that this was correlated with isolates’ innate characteristics, such as biofilm-forming capacity or gene differences.We further chose four isolates for RNA-sequencing analysis and compared their global biofilm transcriptome to their global planktonic transcriptome. Analysis of biofilm vs planktonic gene expression did not show the expected differences in gene expression patterns. Overall, L. monocytogenes persistence in the deli environment is likely a matter of poor sanitation and/or facility design, rather than isolates’ biofilm-forming capacity, sanitizer tolerance, or genomic content
Project description:This study identified and compared the bacterial diversity and the antimicrobial resistance profile of clinically relevant isolates around a newly developed hospital and university precinct
