Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bioinformatic analysis identifies RGS10 as a modulator of BV-2 cell migration

ABSTRACT: Bioinformatic analysis identifies RGS10 as a modulator of BV-2 cell migration

PROVIDER: PRJNA1284326 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Bioinformatic analysis identifies RGS10 as a modulator of BV-2 cell migration

Project description:Regulator of G protein signaling 10 (RGS10) is a GTPase activating protein, selective for Gαi, that has been proposed to play a role in suppressing microglial-driven neuroinflammation. RGS10 expression in microglia is suppressed by inflammatory stimuli and aging, and loss of RGS10 is associated with increased cytokine expression and neurodegeneration. Conversely, RGS10 overexpression provides protection against inflammatory stimuli in rodent models, however the mechanisms by which RGS10 exerts this protective effect are unknown. To understand the neuroprotective functions of RGS10 in microglia, we completed RNA-Seq analysis in the murine microglial cell line BV-2 with intact (BV-2WT) and absent (RGS10-/-) RGS10 expression under basal and IFNγ-treated conditions. RGS10 loss altered expression of 1,579 genes in unstimulated cells and 2,142 genes under IFNγ-stimulated conditions. In addition to changes in GPCR signaling, altered processes in RGS10-/- cells, under basal and stimulated conditions, included cell migration, adhesion, cytokine production and cell development. Key cell adhesion genes such as Mmp9 and Thbs1 were downregulated in RGS10-/- cells regardless of stimuli. Other genes such as Icam1 and Il1b displayed significantly lower expression only in IFNγ-stimulated RGS10-/- cells compared to their WT counterpart. Loss of RGS10 increased BV-2 cell migration, and IFNγ stimulation led to a reduction in migration of both BV-2WT and RGS10-/- cells. The effect of RGS10 on migration could only partially be blocked by the Gαi inhibitor Pertussis toxin (PTX), suggesting involvement of both G protein-dependent and -independent mechanisms. Altogether, these results suggest a novel role for RGS10 in reducing microglial migration through regulation of cell adhesion genes.

2025-07-08 | GSE301244 | GEO

Gene expression profile at single cell level of cells from healthy and BV-induced murine liver.

Project description:Bavachin (BV), a therapeutic phytoestrogen in Fructus Psoraleae (FP), has been used in various diseases. However, the molecular mechanism of BV hepatotoxicity reported by many studies remains unclear. Here, we used single cell RNA sequencing (scRNA-seq) to explore the early cytotoxicity induced by BV in the liver.

2023-08-21 | GSE216336 | GEO

RNA Sequencing Facilitates Quantitative Analysis of BV-2 Transcriptomes

Project description:Using RNA-seq, we report here that BV-2 microglial cells have a distinct transcriptomic signature and express a unique cluster of transcripts in response to 4 hrs LPS.

2016-12-04 | GSE75714 | GEO

FSTL1 promotes keratinocyte migration.

Project description:This study identifies FSTL1 as key component of intiating keratinocyte migration in skin Total RNA from N/TERT-1 keratinocytes transfected with a non-targeting siRNA or Smartpool siRNA against FSTL1 were subjected to microarray analysis

2013-03-02 | E-GEOD-41615 | biostudies-arrayexpress

FSTL1 promotes keratinocyte migration.

Project description:This study identifies FSTL1 as key component of intiating keratinocyte migration in skin

2013-03-02 | GSE41615 | GEO

Alzheimer’s disease-induced phagocytic microglia express a specific profile of coding and non-coding RNAs [BV-2 cells]

Project description:To detect miRNA targets in glioblastoma cell line (BV-2) we stimulate the cells with LPS 10ng/ml or vehicle and then transfect them with miRNA mimics or scrable control We performed differentially expression analysis of RNA-seq BV-2 cells vehicle or LPS stimulated overexpressing miRNA mimic or scramble mimic

2024-03-20 | GSE229416 | GEO

Expression data from microglial cell line BV-2

Project description:Microglial morphology is tightly associated with aspects of their functions during the postnatal stage. It is affected by not only intrinsic cues but also external factors. To pursue the novel factor that controls microglial morphology, we conducted microarray analysis using microglial cell line BV-2. We identified distinct candidates of both up-regulated and down-regulated genes from this experiment.

2020-02-12 | GSE145098 | GEO

MicroRNA-198 an inhibitor of keratinocyte migration

2013-03-02 | GSE37967 | GEO

MicroRNA-198 an inhibitor of keratinocyte migration

Project description:This study identifies miR-198 as a potential inhibitor of keratinocyte migration in skin This study identifies genes differentially expressed during the early phases of wound healing using an ex vivo organ culture model of human skin Total RNA from N/TERT-1 keratinocytes transfected with a negative control miRNA or miR-198 were subjected to microarray analysis Skin from elective plastic surgery were subjected to wounding using ex vivo organ culture model. Total RNA from skin biopsies were isolated at 0hr and 24hr post injury and subjected to mircoarray analysis

2013-03-02 | E-GEOD-37967 | biostudies-arrayexpress

Transcriptome analysis of BV-2 microglial cells displaying a CRISPR/Cas9-induced knockout of peroxisomal genes (Abcd1, Abcd2, Abcd1/Abcd2, Acox1)

Project description:Here, we report the characterization and the comparison of the transcriptomes of BV-2 murine microglial mutant cell lines (CRISPR/Cas9-edited mutations in peroxisomal genes) by RNA-sequencing. Microglia is suspected to play a major role in the neurodegenerative processes observed in peroxisomal leukodystrophies. From CRISPR/Cas9-edited BV-2 microglial cell lines, we aimed at exploring the transcriptomic consequences of a defect of peroxisomal beta-oxidation.

2023-03-28 | GSE200022 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data