Project description:interdigital cell death (ICD) is an essential role in amniotes to promote the regression of interdigital tissue for digits separation. Antagonizing this process can lead to the formation of webbed limbs. However, the mechanism of forming webbed limbs is not the same among independently evolved species, and the reason is still unclear. The Chinese softshell turtle (Pelotiscus sinensis) is a good research model, exhibiting a transitional feature from no webbed to fully webbed. In this study, we collected 8 samples of interdigital webbing from the forelimbs and hindlimbs of turtles at embryonic stage 19 (TK19) for RNA sequencing. We identified 608 potential regulatory genes through differential expression analysis. These findings not only provide new perspectives and data support for comparative studies of adaptive convergent evolution in aquatic animals, but also enhance our understanding of the mechanisms underlying quadrupedal limb morphology. In addition, these results provide potential molecular targets for studying the plasticity of programmed cell death or aging.
Project description:In recent years, the scale culture of Chinese soft-shelled turtle has developed rapidly. However, diseases in aquaculture are the main problems affecting the rapid and healthy cultivation. Strengthening the immunity of Chinese soft-shelled turtles is extremely important to control the infection of pathogenic bacteria. Bacillus has attracted attention as a probiotic supplement in aquatic feeds.In our previous studies, we found that the addition of Bacillus subtilis B10 to diets could increase survival rate, daily weight gain (DG) and feed conversion ratio (FCR) of Chinese soft-shelled turtles, improving the activities of digestive enzyme and optimizing the microbial communities of intestinal in Chinese soft-shelled turtle.However, the study on the mechanism of Bacillus subtilis B10 in Chinese soft-shelled turtle culture remains rare. Therefore, in this study, we used Bacillus subtilis B10 to feed the turtle, and used RNA-seq to explore its mechanism.
