Project description:Crosslinking immunoprecipitation and sequencing was used to characterize nucleocapsid-RNA interactions in Rift Valley fever virus infection. This data set includes illumina HiSeq paired-end reads of Rift Valley fever virus infected HEK293 cells. The sequencing libraries were generated from nucleocapsid-bound RNAs.
Project description:In this study, we used 13C and 15N labeled substrates to assess the carbon (C) and nitrogen (N) assimilation by detecting 13C and 15N in proteins from microorganisms in the phototrophic microbial mats in a soda lake on the Cariboo Plateau, British Columbia, Canada. Metagenomic database used for protein identification and metagenome assembled genome membership had been previousl generated in Zorz et.al. (2019).
Project description:Rift Valley fever virus causes severe disease in humans and livestock and in some cases can be fatal. There is concern about the use of Rift Valley fever virus as a bioweapon since it can be transmitted through the air, and there are no vaccines or antiviral treatments. Airborne transmission of the virus causes severe inflammation of the brain, yet little is known about the immune response against the virus in this organ. Here, we investigated the immune response in the brain to Rift Valley fever virus following intranasal infection. We determined that microglia, the resident immune cells of the brain, initiate a robust response to Rift Valley fever virus infection and identified a key immune pathway that is critical for the ability of microglia to respond to infection. When this immune pathway is rendered non-functional, mice have a dysregulated response to infection in the brain.
Project description:Iso-Seq "full length" transcript sequences were used as one of many guides informing gene model annotation of the Valley Oak genome sequence.
Project description:This study examines the impact of low salinity and temperature on L. redmanii parasitism in Lake Qarun, Egypt, with an emphasis on analyzing the functional gene expression of the parasite, based on RNA sequencing (RNA-Seq). Through the use of de novo transcriptome assembly, this research seeks to identify genes with differential expression in the parasite under different environmental conditions. Thereby, uncovering molecular pathways associated with stress adaptation, metabolism, and virulence.