Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1287346 | ENA |
REPOSITORIES: ENA
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Project description:Meta-proteomics analysis approach in the application of biogas production from anaerobic digestion has many advantages that has not been fully uncovered yet. This study aims to investigate biogas production from a stable 2-stage chicken manure fermentation system in chemical and biological perspective. The diversity and functional protein changes from the 1st stage to 2nd stage is a good indication to expose the differential metabolic processes in anaerobic digestion. The highlight of identified functional proteins explain the causation of accumulated ammonia and carbon sources for methane production. Due to the ammonia stress and nutrient limitation, the hydrogenotrophic methanogenic pathway is adopted as indicative of meta-proteomics data involving the key methanogenic substrates (formate and acetate). Unlike traditional meta-genomic analysis, this study could provide both species names of microorganism and enzymes to directly point the generation pathway of methane and carbon dioxide in investigating biogas production of chicken manure.
2021-02-08 | PXD022498 | Pride
Project description:Metaproteomics analysis of the evolution of protein expression profile during cattle manure composting
2025-01-07 | PXD059527 |
Project description:Reproductive-stage heat stress reduces rice yield and grain appearance quality, even in modern heat-resilient cultivars. Although our previous work showed that green manure can lessen ripening heat damage in the widely grown but heat-susceptible Japanese cultivar Koshihikari, it remains unclear whether similar benefits occur in heat-resilient cultivars and how green manure influences source–sink molecular responses during ripening. Here, using the heat-resilient cultivar Nijinokirameki, we tested whether green manure alleviates ripening heat stress by modifying panicle thermal status, nitrogen assimilation, and early endosperm stress responses. Green manure increased flag-leaf size and tiller (panicle) number and enhanced ripening-stage heat resilience by lowering panicle temperature without a meaningful phenological shift. Consistent with reduced heat load at the target organ, endosperm RNA-seq at 5 days after flowering (DAF) revealed weaker induction of heat-responsive programs under green manure, accompanied by increased grain protein content and reduced chalkiness classes (e.g., white milky, and white belly and back kernels). In parallel, shoot nitrogen contents at panicle formation stage were increased and flag-leaf RNA-seq at 5 DAF showed upregulation of ASN1 and NADH-GOGAT2, suggesting contribution to nitrogen assimilation in source organs and nitrogen allocation to grains. Together, these results link a sustainable fertility practice to tissue-scale heat stress mitigation and coordinated source–sink transcriptional responses at ripening that reflects enhanced grain protein accumulation and appearance quality under green manure treatment.
2026-06-01 | GSE331540 | GEO