Human reproductive system metagenome
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1288139 | ENA |
REPOSITORIES: ENA
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Project description:In postpartum dairy cows, subclinical endometritis (SCE) is characterized by persistent endometrial inflammation, which exerts profound detrimental effects on subsequent reproductive performance. So far, transcriptomic studies related to this condition were either based on biopsy-derived whole endometrium tissue or endometrial swab/cytobrush samples, thus neglecting cell type-specific variations in gene expression. This study tested the hypothesis that different endometrial health statuses are associated with distinct transcription profiles of endometrial stromal, glandular and luminal epithelial cells. In conclusion, this study evidences that endometrial inflammation recovery or persistence is associated with gene expression patterns involved in immune function, tissue remodelling, and uterine receptivity in a cell type-specific manner. Identifying these signatures may prove instrumental to developing novel diagnostic and therapeutic targets, either to prevent persistence or speed recovery from endometrial inflammation, thus restoring the fertility of postpartum dairy cows.
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Project description:Combining the cytological as well as gene expression changes in the endometrium is required to understand the effects of subclinical endometritis on endometrium as well as embryo. Hence, the present study was aimed to investigate the gene expression profiles of subclinical endometrium as well the effect of the inflamed environment on the gene expression profile of the developing preimplantative embryo. Endometrial samples were collected from each 49 cow using the cytobrush technique, 2 h before insemination (Day 0 of the estrous cycle after superovulation) and immediately before flushing (Day 7 of the estrous cycle after superovulation). The endometrial samples were categorized based on the PMN value as healthy endometrium (HE, PMN = 0) and subclinical endometritis (SE, endometrial PMN > 0). Flushed embryos were snap frozen for later molecular genetic analysis. Finally, endometrial samples were pooled according to the endometrial health status of the donor cows (HE vs. SE) at the time of insemination and at the time of flushing. The corresponding samples were subjected global gene expression profile. Moreover embryos flushed from HE and SE cows were pooled together according to the health status of their donors at time of flushing. Those embryos were also used for global embryonic gene expression analysis in relation to the health status of the donor cows.
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