Project description:We constructed three small RNA libraries from embryos at 5, 6 and 7 post-oviposition (hpo) of Bactrocera dorsalis for deep sequencing. The data analysis revealed 147 known and 103 novel miRNAs from these libraries.
Project description:The gut microbiota is considered the host's "second genome" and is closely associated with the host's physiology. We found that the elimination of gut bacteria suppressed ovarian development in Bactrocera dorsalis. Proteomic analysis revealed significant differences in ovarian protein expression after gut bacteria depletion, with differentially expressed proteins enriched in the proteasome and ubiquitin-related pathway. Moreover, ubiquitination levels were significantly reduced in gut bacteria-depleted females, while Enterobacter hormaechei (EH) supplementation rescued ubiquitination levels. Our findings suggest that ubiquitination serves as a mediator through which gut bacteria regulate ovarian development. By ubiquitin-modified proteomic analysis following gut microbiota manipulation and EH supplementation, we aimed to identify ubiquitination targets critical for ovarian development.
Project description:<p>Depletion of gut commensal bacteria significantly reduced nicotinic acid (NA) levels in the hemolymph and ovaries of Bactrocera dorsalis, leading to impaired ovarian development. This inhibition was reversed by exogenous NA supplementation. We identified Enterobacter hormaechei as a key NA-producing gut symbiont. Recolonization with E. hormaechei restored NA levels in both the hemolymph and ovaries, thereby rescuing ovarian development in B. dorsalis.</p>
Project description:<p>Gut commensal bacteria promote host reproduction by modulating metabolism and nutrition, but the molecular mechanisms by which microbes regulate reproduction remain unclear. Here, we show that gut commensal bacteria promote host reproduction by providing amino-acid methionine, which controls the RNA m6A modification level of insulin receptor (InR) in the ovary of the invasive insect Bactrocera dorsalis. Antibiotic-treated B. dorsalis showed reduced RNA m6A methylation levels and methionine content, resulting in arrested ovarian development and decreased fecundity. Gut commensal bacteria Enterobacter hormaechei-derived metabolite methionine restored the decreased RNA m6A level and the reproductive defects. Notably, knockdown of METTL3 and METTL14, two genes encoding the RNA m6A methyltransferases, reduced InR mRNA and protein levels, and impaired ovarian development of in B. dorsalis. Our findings further expand the functional landscape of m6A modification to include nutrient-dependent control of ovarian development and highlight the essential role of epigenetic regulation in microbe-host interactions.</p>
2025-06-22 | MTBLS12470 | MetaboLights
Project description:Gut transcriptome of Bactrocera dorsalis
| PRJNA694509 | ENA
Project description:gut metagenome of Bactrocera dorsalis
Project description:Four developmental stage small RNA libraries including Eggs, Larvae, Pupae and Adults were constructed, where a lot of known miRNAs were identified and many novel miRNAs were predicted. Comparison of their expression profiles in the four libraries suggested that Bactrocera dorsalis miRNAs are dynamically regulated throughout the life cycle.Further analysis of the expression and function of these miRNAs could increase our understanding of regulatory networks in the insect and lead to novel approaches to its control.
