Project description:Arthrobacter chlorophenolicus A6 is a 4-chlorophenol degrading soil bacterium with high phyllosphere colonization capacity. Till now the genetic basis for the phyllosphere competency of Arthrobacter or other pollutant-degrading bacteria is uncertain. We investigated global gene expression profile of A. chlorophenolicus grown in the phyllosphere of common bean (Phaseolus vulgaris) compared to growth on agar surfaces.
Project description:Arthrobacter chlorophenolicus A6 is a 4-chlorophenol degrading soil bacterium with high phyllosphere colonization capacity. Till now the genetic basis for the phyllosphere competency of Arthrobacter or other pollutant-degrading bacteria is uncertain. We investigated global gene expression profile of A. chlorophenolicus grown in the phyllosphere of common bean (Phaseolus vulgaris) compared to growth on agar surfaces. We designed transcriptome arrays and investigated which genes had different transcript levels in the phyllosphere of common bean (Phaseolus vulgaris) as compared to agar surfaces. Since water availability is considered an important factor in phyllosphere survival and activity, we included both high and low relative humidity treatments for the phyllosphere-grown cells. In addition, we determined the expression profile under pollutant exposure by the inclusion of two agar surface treatments, i.e. with and without 4-chlorophenol.
Project description:<p>Plant-microbe interactions in the phyllosphere have been demonstrated to facilitate plant growth and enhance resistance to diverse stresses; nevertheless, their role in the bioaccumulation of contaminants by plants is rarely considered. Here, by comparing differences in the phyllosphere morphology, bacterial community assembly and metabolites between two choysum (Brassica parachinensis) varieties differing in di-n-butyl phthalate (DBP) accumulation, we revealed the pivotal roles of key phyllosphere exudate (fumarate) and recruited specific microbes in determining the variety-specific DBP accumulation in choysum. The low-DBP accumulation variety (LAV) enhanced the recruitment of phyllosphere microbes capable of degrading DBP by increasing fumarate secretion, relative to the high-DBP accumulation variety (HAV), thereby facilitating the colonization of DBP-degrading bacteria via the induction of biofilm formation and ultimately resulting in lower DBP accumulation in leaves. These findings offer novel insights into the LAV formation from the phyllosphere microbial perspective and highlight the role of phyllosphere microbes in mediating pollutant accumulation within crops, which is instrumental in minimizing pollutant accumulation through regulating the phyllosphere microbial community.</p>
Project description:Plants are colonized by a variety of microorganisms, the plant microbiota. In the phyllosphere, the above-ground parts of plants, bacteria are the most abundant inhabitants. Most of these microorganisms are not pathogenic and the plant responses to commensals or to pathogen infection in the presence of commensals are not well understood. We report the Arabidopsis leaf transcriptome after 3 to 4 weeks of colonization by Methylobacterium extorquens PA1 and Sphingomonas melonis Fr1, representatives of two abundant genera in the phyllosphere, compared to axenic plants. In addition, we also sequenced the transcriptome of Arabidopsis 2 and 7 days after spray-infection with a low dose of P. syringae DC3000 and in combination with the commensals.