Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1293400 | ENA |
REPOSITORIES: ENA
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Project description:The most common ladybird beetle, Coccinella septempunctata L., is an excellent predator of crop pests such as aphids and white flies, and it shows a wide range of adaptability, a large appetite and a high reproductive ability. In this study, we collected female adults in three different states, i.e., non-diapause, diapause and diapause termination, for transcriptome sequencing. The experimental insects consisted of three different states as follows: Non-diapause female insects were reared at 24±1°C, with a RH of 70±10% and a 16:8 h light: dark (L: D) photoperiod and collected after their first oviposition. Female adults in diapause were reared at 18±1°C at an RH of 70±10% and a 10:14-h (L:D) photoperiod. The experimental diapause insects were collected after 30 days. Diapause-terminated adults were transferred to another climatic cabinet with the 30-day diapause insects and reared under the same conditions as the non-diapause insects. After their first oviposition, the female insects were collected and stored at -80°C. Three biological replicates per treatment (non-diapause, diapause, diapause-terminated) were sequenced using Illumina HiSeq 2500.
2016-06-01 | E-GEOD-75645 | biostudies-arrayexpress
Project description:Gene expression microarray exprement comparing differential expression between: 1) Early Diapause (ED), 2) Late Diapause (LD), 3) Non-Diapause (ND), 4) Hexane-induced diapause break (HEX).
2010-05-20 | GSE20526 | GEO
Project description:Gene expression microarray exprement comparing differential expression between: 1) Early Diapause (ED), 2) Late Diapause (LD), 3) Non-Diapause (ND), 4) Hexane-induced diapause break (HEX). Four phenotypes (ED,ND,LD,HEX), four replicate pools of four individuals (four individuals in each replicate) in each phenotype, four competitve hybs comparing each phenotype to every other phenotype.
2010-05-20 | E-GEOD-20526 | biostudies-arrayexpress
Project description:Diapause is a key life-history event characterised by arrested development, suppressed metabolism and increased stress tolerance. This state allows an organism to avoid prolonged periods of harsh and inhospitable environmental conditions. For species where only mated female adults undergo diapause, mating is performed prior to diapause. Mating can have a profound effect on the behaviour and physiology of females resulting in changes to the expression of key biological processes, including immunity. However, our understanding of how mating impacts long-term immunity and whether these effects persist throughout diapause is currently limited. Here we explored proteomic changes in the haemolymph of the ecologically important pollinator, Bombus terrestris. B. terrestris queens mate prior to diapause (a non-feeding arrest of development that can last 6-9 months). Using mass-spectrometry-based proteomics, we quantified changes in the pre-diapause queen haemolymph after mating, as well as the subsequent protein expression of mated queens during and post-diapause. Our results provide clear molecular evidence for the consequences and benefits of mating at the immune level through the selective increased abundance of antimicrobial peptides that are sustained throughout diapause. In addition our results provide novel insights into the molecular mechanisms by which bumblebees prepare for, survive, and recover from diapause, insights that may have implications for our general understanding of these processes in other insect groups.
2019-11-18 | PXD014384 | Pride