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Homo sapiens

ABSTRACT: Analysis of HeLa cells after transfection with miR-1 or miR-155, by ribosome profiling and mRNA-Seq

PROVIDER: PRJNA129385 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR057511.fastq.gz	Fastqsanger.gz
	SRR057512.fastq.gz	Fastqsanger.gz
	SRR057513.fastq.gz	Fastqsanger.gz
	SRR057514.fastq.gz	Fastqsanger.gz
	SRR057515.fastq.gz	Fastqsanger.gz

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Mammalian microRNAs predominantly act to decrease target mRNA levels

Project description:This SuperSeries is composed of the following subset Series: GSE21992: Analysis of HeLa cells after transfection with miR-1 or miR-155, by ribosome profiling and mRNA-Seq GSE22001: Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by ribosome profiling and mRNA-Seq GSE22002: Analysis of HeLa cells after transfection with miR-1 or miR-155, by microarray profiling GSE22003: Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by microarray profiling Refer to individual Series

2010-08-02 | E-GEOD-22004 | biostudies-arrayexpress

Analysis of HeLa cells after transfection with miR-1 or miR-155, by ribosome profiling and mRNA-Seq

Project description:MicroRNAs (miRNAs) are endogenous ~22-nucleotide RNAs that mediate important gene-regulatory events by pairing to the mRNAs of protein-coding genes to direct their repression. Repression of these regulatory targets leads to decreased translational efficiency and/or decreased mRNA levels, but the relative contributions of these two outcomes have been largely unknown, particularly for endogenous targets expressed at low-to-moderate levels. Here, we use ribosome profiling to measure the overall effects on protein production and compare these to simultaneously measured effects on mRNA levels. For both ectopic and endogenous miRNA regulatory interactions, lowered mRNA levels account for most (≥84%) of the decreased protein production. These results show that changes in mRNA levels closely reflect the impact of miRNAs on gene expression and indicate that destabilization of target mRNAs is the predominant reason for reduced protein output. Examine ribosome footprints and mRNA abundance of HeLa cells transfected with miR-1 or miR-155, versus mock-transfected cells, at two different time points post-transfection. Supplementary processed data files linked below. mir155_summaryTable.txt: log2 fold changes (miR-155-transfected versus mock-transfected HeLa cells, 32hr). mir1_summaryTable.txt: log2 fold changes (miR-1-transfected versus mock-transfected HeLa cells, 32hr).

2010-08-03 | E-GEOD-21992 | biostudies-arrayexpress

Homo sapiens

Project description:Analysis of HeLa cells after transfection with miR-1 or miR-155, by microarray profiling

| PRJNA129165 | ENA

Analysis of HeLa cells after transfection with miR-1 or miR-155, by microarray profiling

Project description:MicroRNAs (miRNAs) are endogenous ~22-nucleotide RNAs that mediate important gene-regulatory events by pairing to the mRNAs of protein-coding genes to direct their repression. Repression of these regulatory targets leads to decreased translational efficiency and/or decreased mRNA levels, but the relative contributions of these two outcomes have been largely unknown, particularly for endogenous targets expressed at low-to-moderate levels. Here, we use ribosome profiling to measure the overall effects on protein production and compare these to simultaneously measured effects on mRNA levels. For both ectopic and endogenous miRNA regulatory interactions, lowered mRNA levels account for most (≥84%) of the decreased protein production. These results show that changes in mRNA levels closely reflect the impact of miRNAs on gene expression and indicate that destabilization of target mRNAs is the predominant reason for reduced protein output. Examine mRNA expression levels in HeLa cells transfected with miR-1 or miR-155, versus mock-transfected cells, at two different time points post-transfection.

2010-08-03 | E-GEOD-22002 | biostudies-arrayexpress

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2013-12-02 | E-GEOD-52530 | biostudies-arrayexpress

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2015-02-04 | E-GEOD-64364 | biostudies-arrayexpress

hsa-miR-503, hsa-miR-103, and hsa-miR-494 genome wide target profiles [RNA-Seq and RIP-Seq]

Project description:We constructed a genome wide target profile of hsa-miR-503, hsa-miR-103, and hsa-miR-494 by sequencing RNA isolated from Ago2 immunoprecipitations and total RNA samples following transfection of the respective miRNA in mature duplex form Examination of mRNA levels in HeLa cells and Ago2 immunoprecipitations from HeLa cells following miR-503, miR-103, or miR-494 mature duplex or control siRNA transfection

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