Characterization of taste genetics and oral microbiome in severe early childhood caries
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PROVIDER: PRJNA1294420 | ENA |
REPOSITORIES: ENA
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Project description:Characterization of taste genetics and oral microbiome in severe early childhood caries
| PRJNA1294419 | ENA
Project description:Comparative analysis of Severe Early Childhood Caries microbiome
| PRJNA454811 | ENA
Project description:Dental caries is characterized by a dysbiotic shift at the biofilm-tooth surface interface, yet comprehensive biochemical characterizations of the biofilm are scant. We used metabolomics to identify biochemical features of the supragingival biofilm associated with early childhood caries (ECC) prevalence and severity. The study's analytical sample comprised 289 children ages 3 to 5 (51% with ECC) who attended public preschools in North Carolina and were enrolled in a community-based cross-sectional study of early childhood oral health. Clinical examinations were conducted by calibrated examiners in community locations using International Caries Detection and Classification System (ICDAS) criteria. Supragingival plaque collected from the facial/buccal surfaces of all primary teeth in the upper-left quadrant was analyzed using ultra-performance liquid chromatography-tandem mass spectrometry. Associations between individual metabolites and 18 clinical traits (based on different ECC definitions and sets of tooth surfaces) were quantified using Brownian distance correlations (dCor) and linear regression modeling of log<sub>2</sub>-transformed values, applying a false discovery rate multiple testing correction. A tree-based pipeline optimization tool (TPOT)-machine learning process was used to identify the best-fitting ECC classification metabolite model. There were 503 named metabolites identified, including microbial, host, and exogenous biochemicals. Most significant ECC-metabolite associations were positive (i.e., upregulations/enrichments). The localized ECC case definition (ICDAS ≥1 caries experience within the surfaces from which plaque was collected) had the strongest correlation with the metabolome (dCor <i>P</i> = 8 × 10<sup>-3</sup>). Sixteen metabolites were significantly associated with ECC after multiple testing correction, including fucose (<i>P</i> = 3.0 × 10<sup>-6</sup>) and <i>N</i>-acetylneuraminate (p = 6.8 × 10<sup>-6</sup>) with higher ECC prevalence, as well as catechin (<i>P</i> = 4.7 × 10<sup>-6</sup>) and epicatechin (<i>P</i> = 2.9 × 10<sup>-6</sup>) with lower. Catechin, epicatechin, imidazole propionate, fucose, 9,10-DiHOME, and <i>N</i>-acetylneuraminate were among the top 15 metabolites in terms of ECC classification importance in the automated TPOT model. These supragingival biofilm metabolite findings provide novel insights in ECC biology and can serve as the basis for the development of measures of disease activity or risk assessment.
2021-01-29 | MTBLS2215 | MetaboLights
Project description:The development of early childhood caries (ECC) is closely related to the salivary microenvironment, but the role of host factors in the pathogenesis of ECC has not been fully characterized. The aim of this study was to investigate the mechanism of ECC development and to search for salivary protein biomarkers that can predict ECC development by quantitative proteomic analysis of saliva host-derived proteins.
2024-01-17 | PXD048619 |
Project description:Genome-Wide Association Study of Early Childhood Caries: CIDR
| PRJNA671299 | ENA
Project description:Genome-Wide Association Study of Early Childhood Caries: CIDR
| PRJNA671298 | ENA
Project description:<p>Cariogenic microorganisms as well as salivary metabolic status in early childhood caries (ECC) have been reported to differ from that in adult caries. The main aim of this study was to identify specific microbial species and salivary metabolites in children with ECC using species-specific qPCR and untargeted metabolomic approach. Scardovia wiggsiae, Streptococcus mutans, Streptococcus sobrinus, Lactobacillus salivarius and Candida albicans were more prevalent in ECC group. Prevalence of ECC was higher in children with two targeted species present. Histidine metabolism and valine, leucine and isoleucine degradation were activated in ECC group, while glyoxylate and dicarboxylate metabolism, Purine and Pyrimidine metabolism were inhibited. Histidine and glutathione metabolism was activated with enrichment of targeted microorganism, while linoleic acid and biotin metabolism was inhibited. These findings suggest that detection / co-detection of targeted microorganisms can well describe caries status in children. Microbial and metabolite biomarkers can be used in caries control for children.</p>
2024-11-12 | MTBLS10890 | MetaboLights
Project description:Childhood caries is an extremely common childhood chronic disease, affecting 60–90% of children in industrialized countries. It results in lesions in both the primary and permanent dentitions, hospitalizations and emergency room visits, high treatment costs, loss of school days, diminished ability to learn increases the risk of caries in adulthood. Streptococcus mutans is a key bacteria in caries development. While multiple caries risk factors have been identified, significant interpersonal variability not explained by known risk factors still exists. The immune system generates a personal antibody repertoire that helps maintain a balanced and healthy oral microbiome. Using mass-spectrometry, we probed in an hypothesis-free manner which S. mutans proteins are identified by antibodies of children with low and high DMFT (decayed, missing, filled teeth) scores. We identified a core set of proteins, recognized by the immune system of most individuals. This set was enriched with proteins enabling bacterial adhesion, and included glucosyltransferases and glucan-binding proteins known to be important for S. mutans cariogenicity. To explore the physiological relevance of these findings, we tested the ability of saliva from caries free individuals in preventing S. mutans from binding to the tooth surface. Indeed, saliva from individuals with caries free prevented S. mutans binding to teeth. These findings map the S. mutans proteome targeted by the immune system and suggest that inhibiting tooth attachment is a primary mechanism used by the immune system to maintain oral balance and prevent caries. These findings provide new insights into the role of the immune system in maintaining oral health and preventing caries development.
2023-06-26 | PXD043103 | Pride
Project description:To understand the mechanisms that regulate the renewal and maintenance of taste cells we performed RNA-sequencing analysis on isolated taste cells from 2 and 6 month old mice to determine how alterations in the taste cell-transcriptome regulate taste cell maintenance and function in adults. We found that the Activator Protein-1 (AP1) transcription factors (c-Fos, Fosb and c-Jun) and genes associated with this pathway were significantly downregulated in taste cells from 6 month old mice and further declined at 12 months. We generated conditional c-Fos- knockout mice to target K14-expressing cells, including differentiating taste cells. c-Fos deletion caused a severe perturbation in taste bud structure and resulted in a significant reduction in the taste bud size. c-Fos deletion also affected taste cell turnover as evident by, a decrease in proliferative markers, and upregulation of the apoptotic marker cleaved-PARP. Thus, AP1 factors are important regulators of adult taste cell renewal and their downregulation negatively impacts taste maintenance. Comparison of genes responsible for peripheral Taste system maintenance at age, 3 and 6 months. processed_data.txt: List of B vs S gene expression data gene_exp.diff: cuffdiff data for 2 and 6 month taste cells
2016-08-16 | E-GEOD-85308 | biostudies-arrayexpress
Project description:Oral microbiome and HIV in early childhood caries
| PRJEB52879 | ENA