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Promoter G-quadruplex structure as an epigenetic regulator of tissue-specific development and functional formation in Bombyx mori [CUT&Tag]

ABSTRACT: Promoter G-quadruplex structure as an epigenetic regulator of tissue-specific development and functional formation in Bombyx mori [CUT&Tag]

PROVIDER: PRJNA1294731 | ENA |

REPOSITORIES: ENA

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Promoter G-quadruplex structures regulate tissue-specific gene expression and functional differentiation in Bombyx mori [CUT&Tag]

Project description:Cell differentiation, tissue development and functional formation rely on the precise activation of specific gene expression which regulated by the interaction of epigenetic modifications and transcription factors. DNA G-quadruplex structures (G4s) have emerged as important regulators of gene transcription. Here, by nuclear G4-CUT&Tag, ATAC-seq, and histone modification data, we demonstrate that G4s in Bombyx mori are primarily located in promoter regions and are associated with active chromatin marks. Comparative analyses of G4 distribution in the brain and fat body revealed that identical promoter sequences can adopt distinct G4 folding states in different tissues. Notably, the presence of folded G4s strongly correlated with high transcriptional activity of genes essential for tissue-specific functions. Furthermore, treatment with G4-stabilizing ligands disrupted energy metabolism and significantly delayed neurodevelopment in B.mori.. In summary, our findings emphasize G4s as critical epigenetic regulators in maintaining tissue development and functional formation in insects.

2025-09-12 | GSE303268 | GEO

Promoter G-quadruplex structures regulate tissue-specific gene expression and functional differentiation in Bombyx mori [RNA-seq]

Project description:Cell differentiation, tissue development and functional formation rely on the precise activation of specific gene expression which regulated by the interaction of epigenetic modifications and transcription factors. DNA G-quadruplex structures (G4s) have emerged as important regulators of gene transcription. Here, by nuclear G4-CUT&Tag, ATAC-seq, and histone modification data, we demonstrate that G4s in Bombyx mori are primarily located in promoter regions and are associated with active chromatin marks. Comparative analyses of G4 distribution in the brain and fat body revealed that identical promoter sequences can adopt distinct G4 folding states in different tissues. Notably, the presence of folded G4s strongly correlated with high transcriptional activity of genes essential for tissue-specific functions. Furthermore, treatment with G4-stabilizing ligands disrupted energy metabolism and significantly delayed neurodevelopment in B. mori. In summary, our findings emphasize G4s as critical epigenetic regulators in maintaining tissue development and functional formation in insect.

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Promoter G-quadruplex structures regulate tissue-specific gene expression and functional differentiation in Bombyx mori [ATAC-seq]

Project description:Cell differentiation, tissue development and functional formation rely on the precise activation of specific gene expression which regulated by the interaction of epigenetic modifications and transcription factors. DNA G-quadruplex structures (G4s) have emerged as important regulators of gene transcription. Here, by nuclear G4-CUT&Tag, ATAC-seq, and histone modification data, we demonstrate that G4s in Bombyx mori are primarily located in promoter regions and are associated with active chromatin marks. Comparative analyses of G4 distribution in the brain and fat body revealed that identical promoter sequences can adopt distinct G4 folding states in different tissues. Notably, the presence of folded G4s strongly correlated with high transcriptional activity of genes essential for tissue-specific functions. Furthermore, treatment with G4-stabilizing ligands disrupted energy metabolism and significantly delayed neurodevelopment in B. mori. In summary, our findings emphasize G4s as critical epigenetic regulators in maintaining tissue development and functional formation in insect.

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Bombyx mori Posterior and Median Silk Gland SAGE transcriptome

Project description:To identify functions that distinguish the posterior and median cells producing fibroin and sericin in the silk gland of Bombyx mori, serial analysis of gene expression (SAGE) profiles from both silk gland regions were analyzed and compared. The construction of a B. mori reference tag collection extracted from a set of 38000 Bombyx EST sequenced from the 3’ side, helped annotating the SAGE libraries. Most of the tags appeared at similar relative concentration in the two libraries except for those corresponding to silk proteins that were found region-specific and highly abundant. Strikingly, besides tags from silk protein mRNAs, 19 tags were found in the class of high abundance in the median cell library, which were absent in the posterior cell tag collection. Except tags from SP1 mRNA, no PSG specific tags were found in the same class of abundance. The analysis of MSG-specific different transcripts led to suggest that middle silk gland cell realizes more diversified functions as those already known, of synthesis and secretion of the silk sericins.

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