Project description:Sulfate-reducing bacteria (SRB) play a pivotal role in the global carbon- and sulfur cycles, especially in the marine environment. Here, continental margins, coastal ranges, and shelf sediments stand out by their high input of organic matter, and more than 50% of their mineralization is achieved in the upper sediment layers, coupled to sulfate reduction. This turnover is mainly achieved by members of the family of Desulfobacteraceae of completely oxidizing SRB. Desulfonema limicola is a member of this family.
Project description:Sulfate-reducing bacteria (SRB) play a pivotal role in the global carbon- and sulfur cycles, especially in the marine environment. Here, continental margins, coastal ranges, and shelf sediments stand out by their high input of organic matter, and more than 50% of their mineralization is achieved in the upper sediment layers, coupled to sulfate reduction. This turnover is mainly achieved by members of the family of Desulfobacteraceae of completely oxidizing SRB. Desulfonema magnum is a member of this family.
Project description:Sulfate-reducing bacteria (SRB) play a pivotal role in the global carbon- and sulfur cycles, especially in the marine environment. Here, continental margins, coastal ranges, and shelf sediments stand out by their high input of organic matter, and more than 50% of their mineralization is achieved in the upper sediment layers, coupled to sulfate reduction. This turnover is mainly achieved by members of the family of Desulfobacteraceae of completely oxidizing SRB. Desulfobacterium autotrophicum HRM2 is a member of this family.
Project description:Sulfate-reducing bacteria (SRB) play a pivotal role in the global carbon- and sulfur cycles, especially in the marine environment. Here, continental margins, coastal ranges, and shelf sediments stand out by their high input of organic matter, and more than 50% of their mineralization is achieved in the upper sediment layers, coupled to sulfate reduction. This turnover is mainly achieved by members of the family of Desulfobacteraceae of completely oxidizing SRB. Desulfococcus multivorans 1be1 is a member of this family.
Project description:Sulfate-reducing bacteria (SRB) play a pivotal role in the global carbon- and sulfur cycles, especially in the marine environment. Here, continental margins, coastal ranges, and shelf sediments stand out by their high input of organic matter, and more than 50% of their mineralization is achieved in the upper sediment layers, coupled to sulfate reduction. This turnover is mainly achieved by members of the family of Desulfobacteraceae of completely oxidizing SRB. Desulfobacula toluolica Tol2 is a member of this family.
Project description:Sulfate-reducing bacteria (SRB) play a pivotal role in the global carbon- and sulfur cycles, especially in the marine environment. Here, continental margins, coastal ranges, and shelf sediments stand out by their high input of organic matter, and more than 50% of their mineralization is achieved in the upper sediment layers, coupled to sulfate reduction. This turnover is mainly achieved by members of the family of Desulfobacteraceae of completely oxidizing SRB. Desulfosarcina variabilis 3be13 is a member of this family.
2025-03-11 | PXD053571 | Pride
Project description:Mangrove sediments before and after sulfate regulation
Project description:The arterial endothelium’s response to its flow environment is critical to vascular homeostasis. The endothelial glycocalyx has been shown to play a major role in mechanotransduction, but the extent to which the components of the glycocalyx affect the overall function of the endothelium remains unclear. The objective of this study was to further elucidate the role of heparan sulfate as a mechanosensor on the surface of the arterial endothelium, by (1) expanding the variety of shear waveforms investigated, (2) continuously suppressing heparan sulfate expression rather than using a pre-flow batch treatment, and (3) performing microarray analysis on post-flow samples. Porcine aortic endothelial cells were exposed to non-reversing, reversing, and oscillatory shear waveforms for 24 hours with or without continuous heparan sulfate suppression with heparinase. All shear waveforms significantly increased the amount of heparan sulfate on the surface of the endothelium. Suppression of heparan sulfate to less than 25% of control levels did not inhibit shear-induced cell alignment or nitric oxide production, or alter gene expression, for any of the shear waveforms investigated. We infer that heparan sulfate on the surface of porcine aortic endothelial cells is not the primary mechanosensor for many shear-responsive endothelial cell functions in this species.
Project description:The deep marine subsurface is one of the largest unexplored biospheres on Earth, where members of the phylum Chloroflexi are abundant and globally distributed. However, the deep-sea Chloroflexi have remained elusive to cultivation, hampering a more thorough understanding of their metabolisms. In this work, we have successfully isolated a representative of the phylum Chloroflexi, designated strain ZRK33, from deep-sea cold seep sediments. Phylogenetic analyses based on 16S rRNA genes, genomes, RpoB and EF-tu proteins indicated that strain ZRK33 represents a novel class within the phylum Chloroflexi, designated Sulfochloroflexia. We present a detailed description of the phenotypic traits, complete genome sequence and central metabolisms of the novel strain ZRK33. Notably, sulfate and thiosulfate could significantly promote the growth of the new isolate, possibly through accelerating the hydrolysis and uptake of saccharides. Thus, this result reveals that strain ZRK33 may play a crucial part in sulfur cycling in the deep-sea environments. Moreover, the putative genes associated with assimilatory and dissimilatory sulfate reduction are broadly distributed in the genomes of 27 metagenome-assembled genomes (MAGs) from deep-sea cold seep and hydrothermal vents sediments. Together, we propose that the deep marine subsurface Chloroflexi play key roles in sulfur cycling for the first time. This may concomitantly suggest an unsuspected availability of sulfur-containing compounds to allow for the high abundance of Chloroflexi in the deep sea.
Project description:We studied the relation between growth rate and genomewide gene expression, cell cycle progression, and glucose metabolism in 36 steady state continuous cultures limited by one of six different nutrients (glucose, ammonium, sulfate, phosphate, uracil or leucine). The expression of more than a quarter of all yeast genes is linearly correlated with growth rate, independently of the limiting nutrient. The subset of negatively growth-correlated genes is most enriched for peroxisomal functions, whereas positively correlated genes mainly encode ribosomal functions. Many (not all) genes associated with stress response are strongly correlated with growth rate, as are genes that are periodically expressed under conditions of metabolic cycling. We confirmed a linear relationship between growth rate and the fraction of the cell population in the G0/G1 cell cycle phase, independent of limiting nutrient. Cultures limited by auxotrophic requirements wasted excess glucose, whereas those limited on phosphate, sulfate or ammonia did not; this phenomenon (reminiscent of the "Warburg effect" in cancer cells) was confirmed in batch cultures. Using an aggregate of gene expression values, we predict (in both continuous and batch cultures) an "instantaneous growth rate". This concept is useful in interpreting the systemlevel connections among growth rate, metabolism, stress and the cell cycle. Keywords: growth condition design