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ABSTRACT:
PROVIDER: PRJNA1295093 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:To further knowledge of piglet maturity, we have developed a microarray analysis to describe biological processes and to find candidate genes for key roles in piglet maturity. The objective was to identify which genes and biological processes are specifically involved in the difference between two extreme breeds: Large White (LW) and Meishan (MS). The LW breed is a selected breed known to show an increased rate of mortality at birth, while the MS breed presents more robust piglets at birth. MS and LW sows were inseminated with mixed semen (LW and MS) hence each litter was composed of pure fetuses (LW or MS) and crossed fetuses (LWMS from MS sows and MSLW from LW sows).
2017-06-01 | GSE90980 | GEO
Project description:To further knowledge of piglet maturity, we have developed a microarray analysis to describe biological processes and to find candidate genes for key roles in piglet maturity. The objective was to identify which genes and biological processes are specifically involved in the difference between two extreme breeds: Large White (LW) and Meishan (MS). The LW breed is a selected breed known to show an increased rate of mortality at birth, while the MS breed presents more robust piglets at birth. MS and LW sows were inseminated with mixed semen (LW and MS) hence each litter was composed of pure fetuses (LW or MS) and crossed fetuses (LWMS from MS sows and MSLW from LW sows).
2014-09-19 | GSE56301 | GEO
Project description:Whole-genome sequencing of Large White x Landrace reproductive sows
| PRJNA823889 | ENA
Project description:Purpose:The goal of this study was to enrich understanding of the reproductive difference Methods: Multiparous Canadian Large White cyclic sows were divided into two parts: high (H; total number of piglets born > 15.73) and low (L; total number of piglets born < 11.11) fecundity. Eight sows with similar parity from each part were chosen (n=16). Ovarian tissues were obtained on the 14 day (day 1 = first day of estrus) after estrus as in the luteal phase (L) and 20 day of the estrous cycle as in the follicular phase (F). Transcriptome profiling of ovarian tissues were generated by deep sequencing, in quadruplicate, using Illumina HiSeq X10 instrument. Results: Using an optimized data analysis workflow, we obtained the differentially expressed miRNAs between the high and low fecundity, with a fold change ≥1.5 and p value < 0.05. These results provide further insight into fecundity in pigs. Conclusions: Our study represents the first detailed analysis of ovary transcriptome. It will be significantly helpful to display a novel regulatory mechanism for further investigation of prolificacy in pigs.
2020-09-02 | GSE132307 | GEO
Project description:The Chinese Erhualian is one of the most prolific pig breeds in the world, which farrows at least five more piglets per litter than Western pig breeds partly due to a greater ovulation rate. Differences in the transcriptome of Chinese Erhualian and Large White ovaries directly result in variation of ovulation rate. To understand the molecular basis related to ovulation rate in Chinese indigenous and Western breeds, samples were collected and used to hybridized. This study reveals many potential avenues of investigation for seeking new insights into ovarian physiology and the genetic control of reproduction. Expression profiling experiments were conducted to identify differentially expressed genes in ovarian follicles at the preovulatory stage of a PMSG-hCG stimulated estrous cycle from 3 Chinese Erhualian and 3 Large White cycling sows by using the Affymetrix Porcine Genechip™.
2011-12-31 | E-GEOD-23985 | biostudies-arrayexpress
Project description:In this study we had two primary aims: 1.) spatially define the transcriptional signatures of porcine maternal-fetal interface and 2.) develop and validate an organoid model which better recapitulated the porcine placenta. Using mid-gestation maternal-fetal interfaces of commercial landrace/large white composite gilts we performed spatial transcriptomics (n=4 interfaces) using Visium v1 spatial transcriptomics. We then went on to isolate trophoblast organoids from fresh-term placentas from crossbred sows consisting</p><p>655 of Yorkshire, Large White, and Landrace breeds. We then characterized the transcriptional profile of these organoids using bulk RNA-seq from 3 seperate lines using a standard Illumina library preparation. To characterize these organoids we performed single cell RNA-sequencing on 3 separate lines of swine trophoblast organoids using a standard 10x Genomics Single Cell 3' Gene Expression platform. All reads/samples were mapped to Sus scrofa v11.1.
2025-05-13 | GSE296975 | GEO
Project description:n this study we had two primary aims: 1.) spatially define the transcriptional signatures of porcine maternal-fetal interface and 2.) develop and validate an organoid model which better recapitulated the porcine placenta. Using mid-gestation maternal-fetal interfaces of commercial landrace/large white composite gilts we performed spatial transcriptomics (n=4 interfaces) using Visium v1 spatial transcriptomics. We then went on to isolate trophoblast organoids from fresh-term placentas from crossbred sows consisting</p><p>655 of Yorkshire, Large White, and Landrace breeds. We then characterized the transcriptional profile of these organoids using bulk RNA-seq from 3 seperate lines using a standard Illumina library preparation. To characterize these organoids we performed single cell RNA-sequencing on 3 separate lines of swine trophoblast organoids using a standard 10x Genomics Single Cell 3' Gene Expression platform. All reads/samples were mapped to Sus scrofa v11.1.
2025-07-02 | GSE301436 | GEO
Project description:Purpose:The goal of this study was to enrich understanding of the reproductive difference Methods:Multiparous Canadian Large White cyclic sows were divided into two parts: high (H; total number of piglets born > 15.73) and low (L; total number of piglets born < 11.11) fecundity. Eight sows with similar parity from each part were chosen (n=16). Ovarian tissues were obtained on the 14 day (day 1 = first day of estrus) after estrus as in the luteal phase (L) and 20 day of the estrous cycle as in the follicular phase (F). Transcriptome profiling of ovarian tissues were generated by deep sequencing, in quadruplicate, using Illumina HiSeq X10 instrument. Results: Using an optimized data analysis workflow, we obtained the differentially expressed RNAs between the high and low fecundity, with a fold change ≥1.5 and p value < 0.05. These results provide further insight into fecundity in pigs. Conclusions: Our study represents the first detailed analysis of ovary transcriptome. It will be significantly helpful to display a novel regulatory mechanism for further investigation of prolificacy in pigs.
2020-09-02 | GSE134001 | GEO
Project description:To further knowledge of piglet maturity, we have developed a microarray analysis to describe biological processes and to find candidate genes for key roles in piglet maturity. The objective was to identify which genes and biological processes are specifically involved in the difference between two extreme breeds: Large White (LW) and Meishan (MS). The LW breed is a selected breed known to show an increased rate of mortality at birth, while the MS breed presents more robust piglets at birth. MS and LW sows were inseminated with mixed semen (LW and MS) hence each litter was composed of pure fetuses (LW or MS) and crossed fetuses (LWMS from MS sows and MSLW from LW sows). To assay for changes in gene expression during piglet maturity, mRNA was isolated from 61 fetal skeletal muscle samples with 8 different conditions: two fetal gestational ages (90 or 110 days of gestation), associated with four genotypes (two extreme breeds for mortality at birth (MS and LW) and two crossed breeds (MSLW and LWMS)). MS fetuses are known to have a better survival at birth than LW fetuses. An understanding of maturity is possible with the comparison between the fetal gestational ages and between the two extreme breeds. The impact of parental genotypes is studied with the presence of crossed fetuses. After quality control and normalization, only 61 samples were conserved (represented here).
2014-09-19 | E-GEOD-56301 | biostudies-arrayexpress