Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1296676 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Analysis of microbial communities formed in the samples from "duckweed-microbes co-cultivation system"
| PRJDB17002 | ENA
Project description:Purpose:In order to elucidate the molecular mechanism of the effects of different diets on the antioxidant capacity of fish, three groups of adult common carp (Cyprinus carpio) (initial weight 517.8 ±50 g) were fed with earthworm, earthworm + duckweed and duckweed, respectively, which were performed by miRNA sequencing. Methods:MiRNA profiles of common carp fed with earthworm, earthworm + duckweed and duckweed were generated by deep sequencing, in duplicate, using Illumina Hiseq 2500 platform. qRT–PCR validation was performed using SYBR Green assays Results: We built microRNA libraries by using total RNA and contained filtered 20,868,000, 18,984,242, 22,300,487, 19,538,683, 17,110,676 and 14,747,090 high-quality reads that were obtained from sample A1, A2, M1, M2, P1 and P2 respectively.In total, we discovered 236 known carp miRNAs (225, 225, 222, 227, 228 and 219 in A1, A2, M1, M2, P1and P2, respectively).For the known miRNA, 9 differentially expressed miRNAs (DEM) were found between A and M and 8 known miRNAs differentially expressed between P and M, and 11 of these were validated with qRT–PCR. The expression level of 11 known microRNAs via miRNA-seq had highly similar with the relative expression level of 11 known microRNAs via qRT-PCR. Conclusions: In present study, we demonstrated the transcriptional profiles, identification of 5 differentially expressed miRNAs be reported related to oxidative stress, including miR-137-3p, miR-143-3p, miR-146a-5p, miR-21-5p and miR-125b-5p. For omnivorous fish, feed a single bait may might cause oxidative stress inordinately.
2018-07-14 | GSE117059 | GEO
Project description:Complete genome sequence of Polyangiales strain MRC-1 isolated through the duckweed-microbes co-cultivation method
| PRJDB20509 | ENA
Project description:The genome structrure of domesticated species is influenced by complexity of breeding practices exercised by humans. Hokkaido is the northern-most regio of Japan, and one of northern limit of rice cultivation of world. The climatic conditions of Hokkaido are considered to be unsuitable for rice cultivation. Rice breeding programs of Hokkaido have focused on adaptability to specific local environmental condiitons (such as short growth period, low temperature conditions). These specific selection pressures have generated the unique genetic structures of Hokkaido rice cultivars. The genotype of sixty-three Hokkaido rice varieties were already analyzed by SSR marker, and the results showed that Hokkaido rice varieties were classified into six groups (Shinada et al, 2014). The unique genomic structures of six groups may have related to specific gene expression. This study analyze the gene expression profiles of Hokkaido rice variety.
2014-10-31 | GSE57645 | GEO
Project description:Leptochloa chinensis transcriptome changes in response to co-cultivation with rice
| PRJNA744192 | ENA
Project description:To identify the concurrent effects of multiple environmental factors on gene expression under natural climatic fluctuations in rice, we examined global gene expression patterns in two fields typical of the main modes of rice cultivation, over two seasons, each comprising 15 time-points in three different genotypes
2015-10-01 | GSE73609 | GEO
Project description:ONAC127 and ONAC129 are NAC transcription factors that involved in abiotic stress response and play key regulatory roles in apoplasmic transportation during rice caryopsis filling. To reveal the transcription regulatory network of ONAC127 and ONAC129 in rice caryopsis, we performed genome-wide identification of ONAC127 and ONAC129 targets by immunoprecipitation sequencing (ChIP-seq) analyses in the overexpression lines of ONAC127 and ONAC129 under natural heat stress(H) and normal cultivation condition(N) with three technical replicates.
2020-03-26 | GSE140165 | GEO
Project description:To investigate the root system, three indoor cultivation systems, including water agar, deep water, and vermiculite-based hydroponics, are commonly used. However, their effects on the root development of rice (Oryza sativa L.) remain unknown. In this study, we assessed the effect of these indoor cultivation systems on the maximum root length (MRL) and examined the changes in phosphorylated protein profiles by LC-MS/MS.
2024-09-28 | PXD050876 | JPOST Repository
Project description:Mining of fungal genomes uncovered their great potential for the production of novel secondary metabolites (SMs). However most of them stay silent under standard laboratory cultivation conditions. Co-cultivation of fungi with organism that occur in their natural habitat has shown to be trigger for the activation of such silent SM gene clusters. Recently, we showed that the cultivation of Aspergillus nidulans with the bacterium Streptomyces rapamycinicus leads to the activation of the orsellinic acid gene cluster. Hence we decided to study this interaction further to gain insight into the regulation of SM gene clusters and more specifically to study the chromatin remodelling network actuve upon co-cultivation of the two organisms. This study gives novel insight into the regulation of the orsellinic acid gene cluster and the interaction of the two organisms. To the best of our knowledge this is the first report of mapping the chromatin landscape of microbial interactions, making this study a role model for the analysis of similar systems.
2018-10-12 | E-MTAB-5819 | biostudies-arrayexpress
Project description:Improvement of the "Duckweed-Microbes Co-Cultivation Method" for Stable and Efficient Isolation of Rarely Cultivated Bacteria Using Microfilter Membranes
| PRJDB18319 | ENA