Project description:Transcript-Level Variation in Barley Seedling Leaves Challenged with Puccinia hordei and the Molecular Basis of Partial Resistance to Leaf Rust
Project description:Transcript-Level Variation in Barley Seedling Leaves Challenged with Puccinia hordei and the Molecular Basis of Partial Resistance to Leaf Rust
Project description:Time course: Interaction of Puccinia hordei with Hordeum vulgare, Ingrid (leaf) and Puccinia triticana with Hordeum vulgare, Ingrid (leaf)
Project description:Japonica rice cultivar Nipponbare was inoculated with wheat leaf rust (Puccinia triticina f. sp. tritici, non-host pathogen to rice) to compare gene expression profiles with mock-inoculated controls. Although eventually failed in invasion, leaf rust induced a set of rice genes that were distinctally up-regulated, some of those were confirmed by quantitative real-time PCR assays.
Project description:Puccinia graminis f. sp. tritici is the cause of wheat stem rust. A microarray was designed from genes predicted from the P. graminis f. sp. tritici genome assembly, and gene expression measured for four conditions which include wheat or barley infecting growth stages initiated by urediniospores. mRNA was prepared from fresh urediniospores, uredinospores germinated for 24 hr, wheat seedlings infected with urediniospores for 8 days, and barley seedlings infected with urediniospores for 8 days. The asexual uredinial infection cycle on wheat produces additional urediniospores, which can start new cycles of wheat infection and are readily spread by aerial transport. This expression data is further described in Duplessis et al, Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici
Project description:Switchgrass (Panicum virgatum) can be infected by the rust pathogen (Puccinia novopanici) resulting in lowering biomass yields and quality. Here, two cultivars with divergent rust resistance were used to evaluate the dynamic changes in proteomes following rust inoculation. Label-free quantitative proteomics was conducted on leaf extracts harvested from a susceptible cultivar Summer at 7, 11, and 18 DAI, and leaves collected at 18 DAI from controls and infected plants of the more resistant cultivar Kanlow.
Project description:Transcriptomic profiling was done on barley samples incoulated with different Puccinia graminis f. sp. tritici (Pgt) isolates of varying virulence profile on barley stem rust resistance gene rpg4/5 and Rpg1. Several differentally expressed host and pathogen genes were identified while comparing gene expression profiles between group of samples inoculated with isoaltes of varying virulence. Several variants with genes of pathogen were idnetified that are possibly associated with virulence to rpg4/5.
Project description:Dikaryotic rust fungi maintain two distinct haploid nuclei for most of their life cycle, making their large, repeat-rich genomes difficult to assemble and phase. Here we present haplotype-phased, near chromosome-scale genome assemblies for the poplar rust pathogens Melampsora larici-populina 98AG31 and Melampsora allii-populina 12AY07, generated using PacBio HiFi sequencing and Hi-C-guided scaffolding. For each species, we resolve 18 chromosomes per haplotype, providing the first chromosome-level representations of poplar rust fungal species. M. larici-populina diploid assembly spans ~203 Mb, while M. allii-populina reaches ~416 Mb, with high completeness and strong collinearity between haplotypes.
