Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1299282 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Green manure is widely advocated as a sustainable alternative to chemical fertilizers in crop systems, yet the mechanisms underlying its yield benefits remain unclear. Moreover, vigorous vegetative growth under green manure can elevate lodging risk, undermining yield and harvest efficiency. Here, we describe mechanisms by which hairy vetch–based green manure enhances yield and evaluate the practical value of deploying functionally weak alleles of gibberellin 20-oxidase (GA20ox) in this management context. We conducted field comparisons of green manure and conventional chemical fertilization to evaluate effects on rice productivity, grain appearance quality, and canopy physiology. Green manure significantly increased grain yield and grain appearance quality in the leading Japanese cultivar ‘Koshihikari’, accompanied by higher lodging. By contrast, high-yielding cultivars homozygous for a single-copy GA20ox1 allele and/or a non-functional GA20ox2 allele maintained superior lodging resistance under green manure treatment while improving yield and grain appearance quality, indicating an effective combination of its treatment and genotypes. Physiologically, green manure increased chlorophyll index during vegetative growth and at the reproductive stage, and nitrogen (N) concentration on the whole plant. Furthermore, green manure increased flag-leaf width and tiller number; these canopy changes were associated with reduced panicle temperature at the ripening stage. Green manure treatment induced upregulation of OsNADH-GOGAT2, a known gene associated with increased N loading to grains, and more grain storage proteins, providing a positive link to improved grain appearance quality. Collectively, this study demonstrates that integrating hairy vetch with functionally weak GA20ox alleles can enhance productivity and grain appearance quality while mitigating lodging risk. This sheds light on the importance of aligning green-manure treatment with targeted allelic selection to stabilize performance across intensive-farming systems and reduce chemical fertilizer dependency.
2026-01-19 | GSE313855 | GEO
Project description:Intercropping is a vital technology in resource-limited agricultural systems with low inputs. Peanut/maize intercropping enhances iron (Fe) nutrition in calcareous soil. Proteomic studies of the differences in peanut leaves, maize leaves and maize roots between intercropping and monocropping systems indicated that peanut/maize intercropping not only improves Fe availability in the rhizosphere but also influences the levels of proteins related to carbon and nitrogen metabolism. Moreover, intercropping may enhance stress resistance in the peanut plant (Xiong et al. 2013b). Although the mechanism and molecular ecological significance of peanut/maize intercropping have been investigated, little is known about the genes and/or gene products in peanut and maize roots that mediate the benefits of intercropping. In the present study, we investigated the transcriptomes of maize roots grown in intercropping and monocropping systems by microarray analysis. The results enabled exploration differentially expressed genes in intercropped maize. Peanut (Arachis hypogaea L. cv. Luhua14) and maize (Zea mays L. cv. Nongda108) seeds were grown in calcareous sandy soil in a greenhouse. The soil was enhanced with basal fertilizers [composition (mg·kg−1 soil): N, 100 (Ca (NO3)2·4H2O); P, 150 (KH2PO4); K, 100 (KCl); Mg, 50 (MgSO4·7H2O); Cu, 5 (CuSO4·5H2O); and Zn, 5 (ZnSO4·7H2O)]. The experiment consisted of three cropping treatments: peanut monocropping, maize monocropping and intercropping of peanut and maize. After germination of peanut for 10 days, maize was sown. Maize samples were harvested after 63 days of growth of peanut plants based on the degree of Fe chlorosis in the leaves of monocropped peanut. The leaves of monocropped peanut plants exhibited symptoms of Fe-deficiency chlorosis at 63 days, while the leaves of peanut plants intercropped with maize maintained a green color.
2017-01-24 | GSE93771 | GEO
Project description:<p>Common vetch (Vicia sativa L.) is an important annual leguminous forage crop commonly used for green manure, fodder, and soil improvement. It is widely cultivated as a green manure and forage crop in Yunnan Province during winter and spring. However, the dry conditions and minimal rainfall during these seasons greatly limit common vetch growth. Therefore, screening for drought-tolerant, high-yielding common vetch varieties is a critical objective in breeding programs.</p>
2026-01-31 | MTBLS13368 | MetaboLights
Project description:<p>Soil-borne diseases, with their high incidence and frequency in monoculture systems, pose a major challenge in contemporary agricultural production. Intercropping can promote beneficial soil legacy effects, thereby effectively mitigating the occurrence and damage of soil-borne diseases. In this study, we employed an integrated approach combining 16S rRNA sequencing, ITS amplicon sequencing, and untargeted metabolomics to systematically compare the differences in soil microbial community structure and metabolite profiles between soybean-tobacco intercropping and tobacco monoculture systems. Furthermore, we elucidated the mechanisms through which these differences influence the incidence of tobacco root rot. The results showed that intercropping significantly enhanced the survival rate of tobacco plants under Fusarium.spp infection (P < 0.01). Furthermore, intercropping markedly increased soil microbial community diversity and significantly reduced the relative abundance of Fusarium (by 53.17%). Additionally, intercropping disrupted the cooperative relationships between Fusarium and other microbial taxa, leading to reduced connectivity within the interaction network and a notable decline in its ecological competitive advantage. Metabolomic analysis revealed that intercropping promoted the accumulation of antimicrobial metabolites such as indole, and indole content was significantly negatively correlated with Fusarium abundance (P < 0.05). Further integrated microbiome-metabolome analysis demonstrated that intercropping fostered a more complex microbial-metabolite interaction network, which helped suppress the recolonization of pathogenic Fusarium. In conclusion, this study provides a theoretical basis for leveraging intercropping systems to modulate the rhizosphere micro-environment and control soil-borne diseases, offering new insights for developing sustainable green control strategies.</p>
2025-09-05 | MTBLS12940 | MetaboLights
Project description:Fungal community after green manure incorporation
| PRJDB11369 | ENA