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ABSTRACT:
PROVIDER: PRJNA1299443 | ENA |
REPOSITORIES: ENA
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Project description:In this manuscript, we present a more extensive analysis of inflammatory suppression mediated by L. plantarum at the respiratory tract. Via full genome microarray of whole lung tissue, we have generated an extensive list of soluble proinflammatory mediators that are expressed in response to PVM infection and we identify those mediators that are suppressed and also those that are not suppressed in response to L. plantarum priming. We focused further study on three specific virus-induced soluble mediators that are differentially expressed and that serve as specific biomarkers for Lactobacillus-mediated survival in response to acute respiratory virus infection. Among several novel directions, we use these biomarker cytokines to explore Lactobacillus-mediated actions at the respiratory tract that are unique and distinct from those taking place at gastrointestinal mucosa. innoculation of mouse using combinations of PBS/BSA, Lactobacillus plantarum and pneumonia virus
2015-11-02 | E-GEOD-66721 | biostudies-arrayexpress
Project description:Inflammatory bowel disease (IBD) is characterized by progressive epithelial injury, chronic inflammation, and impaired intestinal barrier function. To comprehensively delineate the epithelial alterations and stress-related responses that occur during colitis progression, we investigated the functional role of integrin beta-like 1 (ITGBL1) in intestinal epithelial cells (IECs). Using patient specimens and DSS-induced colitis models, we identified marked upregulation of ITGBL1 in inflamed intestinal tissues. Loss of ITGBL1 resulted in the emergence of ferroptosis-prone epithelial subsets exhibiting elevated ACSL4 expression, lipid peroxidation, mitochondrial shrinkage, and enhanced oxidative stress, all of which were associated with aggravated epithelial damage and worse inflammatory outcomes. We further demonstrated that ITGBL1 deficiency activated the ANGPT2/PI3K-AKT signaling axis in IECs, leading to exaggerated oxidative stress and ferroptosis, accompanied by reduced expression of key tight-junction proteins including E-cadherin, ZO-1, occludin, and claudin-1. These alterations impaired epithelial cohesion and barrier integrity during disease progression. In contrast, supplementation with recombinant ITGBL1 suppressed ANGPT2 expression, mitigated PI3K-AKT hyperactivation, restored mitochondrial structure, alleviated ferroptosis, and improved epithelial barrier function. In conclusion, ITGBL1 serves as a critical regulator of epithelial stress responses and barrier stability during colitis, acting by restraining ANGPT2-mediated PI3K-AKT activation and ferroptotic cell death. These findings highlight a previously unrecognized epithelial-protective mechanism and identify the ITGBL1–ANGPT2 axis as a potential therapeutic target in IBD.
2025-12-31 | GSE314630 | GEO
Project description:In this study, we examined Caco-2 cell gene expression after infection with E. coli (Ec), Lactobacillus plantarum (Lp) and the combination of the two (mix) Keywords: Lactobacillus plantarum and E. coli influences on Caco2 cells gene expression
2007-09-13 | GSE5874 | GEO
Project description:Mannose-specific interactions of Lactobacillus plantarum 299v with jejunal epithelium were investigated using an in situ pig small intestinal segment perfusion (SISP) model. L. plantarum 299v wildtype strain was compared to two isogenic mutant strains either lacking the gene encoding for the mannose-specific adhesin (msa) or sortase (srtA; responsible for anchoring of cell surface proteins like Msa to the cell wall). Salmonella typhimurium served as a positive control for gene expression analysis. Scrapings from jejunal segments were collected after perfusion with bacterial suspensions or PBS (control) for 4 or 8 hours, and host gene expression was assessed using a home-made cDNA porcine microarray. Keywords: host-microbe interaction, Lactobacillus plantarum, mannose-specific adhesion A Small Intestinal Segment Perfusion (SISP) test was performed using 4 pigs. 10 segments were prepared in the jejunum of each pig and perfused with Lactobacillus plantarum 299v wildtype, Lactobacillus plantarum 299v msa mutant strain, Lactobacillus plantarum 299v srtA mutant strain, Salmonella typhimurium or PBS (control) for 4 or 8 hours. Pooled samples from each treatment at each timepoint were used for microarray analysis. 8 comparisons were done: L. plantarum wildtype vs control (4 hours), L. plantarum wildtype vs control (8 hours), L. plantarum msa mutant vs control (4 hours), L. plantarum msa mutant vs control (8 hours), L. plantarum srt mutant vs control (4 hours), L. plantarum srt mutant vs control (8 hours), S. typhimurium vs control (8 hours), samples taken at the beginning of the experiment vs control (8 hours). Dye-swaps were performed for each comparison.
2010-05-25 | E-GEOD-9209 | biostudies-arrayexpress
Project description:In this experiment we analyzed the impact of the disruption of trxB1in Lactobacillus plantarum at the transcriptome level. Furthermore we studied the effect of 3.5 mM peroxide effect on both Lactobacillus plantarum wild type (strain WCFS1) and a trxB1 mutant (strain NZ7608). Keywords: mutant analysis of trxB1, hydrogen peroxide stress
2007-08-09 | GSE8672 | GEO
Project description:Aim: To assess the effect of IL-26 on mouse colonic tissue at steady state and in DSS-induced colitis through next-generation RNA sequencing of bulk RNA from colonic tissue. Results: We demonstrate an anti-inflammatory effect of IL-26 at both steady-state and after induction of DSS-colitis, through suppression of both cell recruitment and activation pathways.
2020-08-02 | GSE148505 | GEO
Project description:In order to understand LBG derived galacto-manno-oligosaccharides utilization by a probiotic bacterium, Lactobacillus plantarum WCFS1, we have grown Lactobacillus plantarum WCFS1 (in duplicates) till mid log phase (OD600nm ~0.5, 10 h) in carbon free MRS (de Man, Rogosa Sharpe ) media containing either galacto-manno-oligosaccharides, mannose, glucose or galactose (1% w/v) as the sole carbon source.
2019-07-30 | GSE117591 | GEO