Project description:We performed small RNA sequencing (TruSeq) of gene expression on bronchial cells from human bronchial epithelial brushings from 16 independent subjects whose samples were classified as either healthy controls (with no asthma or lung disease) or steroid-naive asthmatics (subjects with asthma not using inhaled corticosteroids (ICS) for 6 weeks before enrollment that were studied at baseline ('Steroid-naive asthma - Baseline') or after 8 weeks of treatment with budesonide, 200 μg twice a day, ('Steroid-naive asthma - Post-ICS treatment')). The goal was to assess abundance of miRNAs in all the samples collectively.
Project description:Single-cell RNA and spatial sequencing of lesional and non-lesional cutaneous lupus skin compared to healthy controls. The study includes 12 single-cell samples (cutaneous lupus patients with non-lesional (5) and lesional (4) skin biopsies with healthy controls (3)) and 4 spatial transcriptomics samples (cutaneous lupus patient pre/post-Anifrolumab treatment, 1 additional CLE patient, and 1 healthy control).
Project description:Genome-wide DNA methylation profiling of nasal DNA from asthmatics hospitlaized for exacerbation. Samples were taken before steroid treatment and 18-24hr after steroid treatment. The Illumina Infinium HumanMethylation450 beadchips were used to generate DNA methylation profiles.
Project description:The heterogeneity of asthma has prompted attempts at classifications based on clinical phenotypes and cluster designations. Although helpful, reliance on clinical parameters fails to address fundamental issues resulting in specific phenotypes, in particular, steroid-resistant (SR) asthma. Limited data are available defining the mechanisms or pathways (endotypes) causing the failure of inhaled corticosteroids (ICS) in SR asthmatics. Unlike CD4+ T cells, both human and mouse CD8+ T cells fail to undergo apoptosis in the presence of corticosteroids (CS) positioning them as unique effector cells. Whole transcriptome analyses of peripheral CD8+ T cells from steroid-sensitive or steroid-resistant asthmatics or healthy controls on days 0 and 8 were performed.
Project description:We collected airway epithelial brushings for microarray analysis from 42 asthmatics and two control groups – 28 healthy subjects and 16 smokers. A subgroup of 32 asthmatics completed a randomized placebo-controlled trial of fluticasone propionate in which collection of brushings was repeated after 1 week of treatment. Keywords: disease state analysis, clinical trial
