Project description:Serinicoccus kebangsaanensis sp. nov strain P2D13-UKM is a new species of Gram-positive bacteria isolated from a toxic diatom, Nitzschia navis-varingica. It is a halophilic aerobic, oxidase-negative, catalase-positive, circular, and colonies with white colour. Based on the 16S rRNA gene, the closest species were Serinicoccus profundi MCCC 1A05965 strain 0714S6-1, 97.41%, and Serinicoccus hydrothermalis strain JLT9, 97.35%. This bacteria's predominant cellular fatty acids were iso-C15: 0 (30.1 %) and iso-C16: 0 (16.2 %). The polar lipids identified in this bacterium were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, and an unknown glycolipid. The whole-genome sequence analysis of strain P2D13-UKM showed less than 85% similarity from other Serinicoccus species. The genomic DNA G + C content is 72.2 %. Here, we report the main characteristic of strain P2D13-UKM as a new species of bacteria according to its draft genome sequence, which was deposited in Gene Bank and is publicly available under the accession number VSLG00000000.
Project description:RNA sequencing (RNA-seq) experiment was performed on 18 samples of Nitzschia laevis cells collected at 3 h, 6 h and 12 h after inoculation in treated medium with glucose addition (+G) and control group (-G) respectively. The de novo assembly totally generated 16650 unigenes.
