Project description:Candida auris has been globally recognized as a multidrug-resistant human fungal pathogen that contributes for the worldwide occurrence of nosocomial outbreaks. It has been reported that C. auris was able to avoid neutrophil attack, suggestive of an impaired innate immune response. Whether C. auris evades the innate immune recognition of BMDM (bone marrow derived macrophage) remains poorly understood, and as for well-known Candida species -C. albicans, it can trigger immune response. To determine whether occurs difference between immune response stimulated by C. auris or C. albicans, we performed mRNA-seq of BMDM stimulated by C. auris or C. albicans.
Project description:Candida auris clinical isolate FY279 was exposed to tebuconazole (32μg/ml). Randomly14 adaptors were chosen. 10 adaptors obtained resistance to tebuconazole. These resistant adaptors were sequenced.
Project description:Approximately 1 million cells of Candida auris isolate FY279 were spread on YPD plate supplemented with 8ug/ml tunicamycin. Randomly 21 adaptors were chosen. These adaptors were sequenced.
Project description:Candida auris reference strain B11221 was spread on YPD plate supplemented with 8 μg/ml tunicamycin. Randomly 25 adaptors (T2080-T2104) were chosen. These adaptors and the parent were sequenced.
