Project description:The transcription factor DMRT1 is a key gene for testis differentiation and male fertility, but less is known about its potential involvement in ovarian differentiation. In mice, Dmrt1-/- females are fertile, even though they show a reduction of Stra8 expression. Using our DMRT1 mutant rabbit model, we analyzed ovarian differentiation when DMRT1 expression is abolished. Firstly, we observed that during early gonadal development, DMRT1-/- gonads present a specific transcriptome different from control testes as well as control ovaries. Secondly, we assessed the infertility phenotype of mutant females. DMRT1 is highly expressed in germ cells until meiosis initiation, and its disruption leads to a downregulation of key genes involved in the meiotic process. ChIP-sequencing data highlighted that DMRT1 can bind these genes in the rabbit ovary. In addition to its role in testicular differentiation, we describe two roles of DMRT1 in ovarian differentiation. Our data suggested that DMRT1 can be considered as a gene necessary for human female fertility.
Project description:In order to explore the mechanisms of Dmrt1 in regulating testicular immune homeostasis, we performed ChIP-seq analysis on primary SCs infected with a lentiviral vector expressing FLAG-tagged Dmrt1
Project description:To explore the potential function of Dmrt1 in spermatogenesis, we constructed a Dmrt1-deficiency mouse model by knockdown Dmrt1 in testicular seminiferous tubule specifically
Project description:Changes in gene expression in the preoptic area/hypothalamus/thalamus of Xenopus tropicalis tadpoles were analyzed using RNAseq at four stages of metamorphosis.
Project description:Dmrt1 (doublesex and mab-3 related transcription factor 1) is a conserved transcriptional regulator of male differentiation required for testicular development in vertebrates. In mice of the 129Sv strain, loss of Dmrt1 causes a high incidence of teratomas. Mutant 129Sv germ cells undergo apparently normal differentiation up to embryonic day 13.5 (E13.5), but some cells fail to arrest mitosis and ectopically express pluripotency markers. Expression analysis and chromatin immunoprecipitation identified DMRT1 target genes whose misexpression may underly teratoma formation. Comparison of E13.5 testes gene expression between Dmrt1(+/-) and Dmrt1(-,-) animals that show high incidence of teratoma formation.
