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Highly active chromosome regions preferentially associate with two perispeckle networks that partition the interchromatin space.

ABSTRACT: Highly active chromosome regions preferentially associate with two perispeckle networks that partition the interchromatin space.

PROVIDER: PRJNA1302778 | ENA |

REPOSITORIES: ENA

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Highly active chromosome regions preferentially associate with two perispeckle networks that partition the interchromatin space (TSA-seq)

Project description:Highly active chromosome regions preferentially associate with two perispeckle networks that partition the interchromatin space (TSA-seq)

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Highly active chromosome regions preferentially associate with two perispeckle networks that partition the interchromatin space (RNA-seq)

Project description:Highly active chromosome regions preferentially associate with two perispeckle networks that partition the interchromatin space (RNA-seq)

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Highly active chromosome regions preferentially associate with two perispeckle networks that partition the interchromatin space (RNA-seq)

Project description:A subset of highly active chromosomal "hot zones" reproducibly positions adjacent to nuclear speckles (NS). Genes within these regions amplify their expression with NS proximity. However, gene expression changes inversely correlate with changes in NS distance genome-wide. We hypothesized the existence of additional gene expression "niches" away from but spatially correlated with NS. Here we report the identification of two dynamic perispeckle patterns of protein concentrations extending outwards from nuclear speckles and persisting even after NS are eliminated. Highly active chromosome regions which weakly associate with NS instead show close, NS-independent association with these perispeckle patterns. Additionally, transcripts from model intron-containing versus intronless genes associate differentially with these two patterns. While genes within speckle-associated genomic regions predominately are downregulated upon nuclear speckle depletion, genes associated with perispeckle patterns are biased towards upregulation. We suggest the interchromatin space (ICS) is partitioned into additional gene expression “niches”- surrounding and extending from nuclear speckles- that may be involved in mRNA and gene dynamics.

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Bacillus subtilis spores, PROTECT experiment, Space-exposed and Mars-exposed vs. Earth-control

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