Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1303007 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Expanded Genome and Proteome Reallocation in a Novel, Robust Bacillus coagulans Capable of Utilizing Pentose and Hexose Sugars
2024-07-03 | MSV000095240 | MassIVE
Project description:A popular strategy for enhancing the antibacterial properties of probiotic bacteria is to retrofit them with the ability to overproduce heterologous bacteriocins. This is often achieved from strong, non-native promoters. How the dysregulated overproduction of heterologous bacteriocins affects the fitness and antibacterial efficacy of the retrofitted probiotic bacteria is often overlooked. We conferred the prototypical probiotic Escherichia coli strain Nissle (EcN) the ability to produce different amounts of the bacteriocin microcin C (McC). Expression of the bacteriocin synthesis genes was driven from the native promoter (Pmcc-WT), or from promoters manipulated to be stronger (Pmcc-High) and weaker (Pmcc-Low) than the WT, in a plasmid-based system. Pmcc-Low and Pmcc-High retained their native regulation. A strain harbouring a non-functional promoter (Pmcc-Mut) produces no McC and was used as a control. Each strain was grown to early stationary phase, when production of McC starts, in Luria-Bertani broth at 37 degrees. The RNA was isolated and the effects of different levels of production of McC on the transcriptome of EcN was examined by RNA-Seq.
2023-06-30 | E-MTAB-13078 | biostudies-arrayexpress
Project description:Bacillus coagulans DSM 1 Genome sequencing
| PRJNA245631 | ENA
Project description:This project provides tandem mass spectrometry datasets of an artificial microbiota composed of 24 microbial strains: Bacillus cereus ATCC 14579, Bacillus subtilis ATCC 6633, Bacillus thuringiensis DSM 5815, Bordetella parapertussis Bpp5, Cellulophaga lytica DSM 7489, Deinococcus deserti VCD115, Deinococcus geothermalis DSM 11300, Deinococcus proteolyticus DSM 20540, Kineococcus radiotolerans SRS30216, Marivirga tractuosa DSM 4126, Oceanibulbus indolifex HEL-45, Oceanicola granulosus HTCC2516, Phaeobacter inhibens DSM 17395, Pseudomonas putida mt-2 KT2440, Pseudopedobacter saltans DSM 12145, Roseobacter denitrificans OCh 114, Roseovarius nubinhibens ISM, Ruegeria pomeroyi DSS-3, Sagittula stellata E 37, Salmonella bongori NCTC 12419, Shigella flexneri 2a 2457T, Sphingomonas wittichii RW1, Staphylococcus carnosus TM300, Vibrio harveyi ATCC 14126
2023-06-02 | PXD005776 | Pride
Project description:This project provides tandem mass spectrometry datasets of an artificial microbiota composed of 24 microbial strains: Bacillus cereus ATCC 14579, Bacillus subtilis ATCC 6633, Bacillus thuringiensis DSM 5815, Bordetella parapertussis Bpp5, Cellulophaga lytica DSM 7489, Deinococcus deserti VCD115, Deinococcus geothermalis DSM 11300, Deinococcus proteolyticus DSM 20540, Kineococcus radiotolerans SRS30216, Marivirga tractuosa DSM 4126, Oceanibulbus indolifex HEL-45, Oceanicola granulosus HTCC2516, Phaeobacter inhibens DSM 17395, Pseudomonas putida mt-2 KT2440, Pseudopedobacter saltans DSM 12145, Roseobacter denitrificans OCh 114, Roseovarius nubinhibens ISM, Ruegeria pomeroyi DSS-3, Sagittula stellata E 37, Salmonella bongori NCTC 12419, Shigella flexneri 2a 2457T, Sphingomonas wittichii RW1, Staphylococcus carnosus TM300, Vibrio harveyi ATCC 14126
2023-06-02 | PXD005759 | Pride