Project description:We have employed a single cell sequencing approach using 10x Genomics scRNAseq to study the single-cell atlas of Pogostemon cablin globular somatic embryos (GSE) and cotyledonary somatic embryos (CSE). Pseudo-time analysis reconstructed the continuous differentiation trajectory of apical meristem cells and epidermal cells. Further research identified PcNAC048 as a potential transcription factor regulating embryonic cell differentiation, which promotes lateral root development and negatively regulates the biosynthesis of patchouli alcohol. Overall, this study provides theoretical support for the development of Pogostemon cablin germplasm resources and the regulation of medicinal compounds.
Project description:Implementation and evaluation of tests (PCR, ELISA, NGS) for the presence of viruses, viroids and phytoplasma in propagation materials of apple. Research and application of new sanitation techniques using promising antiviral substances, or cryo-knife. Confirmation of health status of sanitated cultivars including use of modern diagnostic methods such as high-throughput sequencing (NGS).
2022-12-09 | GSE205183 | GEO
Project description:Key Gene Mining and New Breeding Materials Creation of Pig Characteristic Germplasm Resources
Project description:We selected wild A. venetum of four distributed regions (jinta county, minqin county, hangjinqi and baicheng) as the test materials in the study. Based on the study of community composition, population genetic diversity and soil environmental properties of wild A. venetum, physiological characteristics, transcriptomics and proteomics analysis of A. venetum under salt stress, and key regulatory proteins and genes of A. venetum salt tolerance were measured and identified to reveal the ecological adaptability of wild A. venetum and underlying mechanisms in response to salt stress. The study is beneficial to promote the protection and rational development and utilization of germplasm resources of wild A. venetum, and is of great significance to the development of national pharmacology and the construction of ecological civilization in China.
2024-04-09 | GSE229255 | GEO
Project description:Resequencing of peanut germplasm resources
Project description:Hernandia nymphaeifolia is an endangered mangrove associate with high ecological, ornamental, and medicinal values. Its special combination of glycophytic and halophytic characteristics provides an ideal system for investigating salt-responsive mechanisms that may be easily extended to genetic improvement of crops with high salt tolerance. However, little is known regarding salt-responsive mechanisms in H. nymphaeifolia. In this study, we posed gradient salt treatments on H. nymphaeifolia seedlings and investigated their physiological and transcriptional reprogramming in response to salinity stress. The results revealed that hyper-salinity stress posed more adverse impacts on leaf growth, cell integrity and photosynthetic performance of H. nymphaeifolia seedlings compared to those in growing in fresh water or low salt condition, mirroring its nature as a mangrove associate. Genes associated with osmolarity sensor and regulator, ROS scavenging and ion homeostasis were differentially expressed accordingly to alleviate the destructive effects. Furthermore, our results unraveled some pivotal kinases and proteins that regulate the synergistic expression of salt-responsive genes, which may act a key role in regulating the transcriptional remodeling upon salinity stress. These findings enrich our knowledge on the molecular mechanisms underlying the phenotypic plasticity of mangrove associates, and also provide valuable genetic resources for mangrove’s conservation and potential bioengineering application in agricultural field.
